[HSS1100] - Final Exam Guide - Everything you need to know! (95 pages long)

• School: University of Ottawa
• Department: Health Sciences
• Course: HSS 1100
• Professor: Franco Pagotto

Document Summary

Inoculation methods: streak, spread or pour, streak: most common way of isolating bacteria; you have a petri dish with whatever inside. So that you can look at it later and study it to see what it is. This third step is the most critical and the one most affected by technical variations in timing and reagents: flood with safranin (pink color). (wash with water). Fluorescence microscopy: dye fluoresces at specific wavelength, antibodies tagged with dyes are common (immunofluorescence microscopy) Electron microscopy: electron beam (instead of light, millio(cid:374) ti(cid:373)es (cid:373)ag(cid:374)ifi(cid:272)atio(cid:374) possi(cid:271)le (cid:894)(cid:1004). (cid:1004)(cid:1004)(cid:1007) (cid:373)(cid:895, t(transmission)em (stain with heavy metals, s(scanning)em (3-d image of cell surface) Bottom line: morphology helps classify and identify, gram stain, gives clues to how they behave in the environment, capsules(harder to get to) Characteristics of bacteria: small (0. 75 (cid:1005). (cid:1006)5 (cid:373) i(cid:374) dia(cid:373)ete(cid:396)/(cid:449)idth(cid:895, higher surface area / volume ratio, higher metabolism, faster growth, replication rate (~20 minutes)