Expression vectors inprokaryotes do not make functional eukaryotic gene products inbacteria very well because
Answer Not sure which one ?!
| | · the codon sequence for prokaryotes is differentthan the codon sequence in eukaryotes |
| | · there are no disulfide bridges formed in proteinsnormally made in prokaryotes |
| | · prokaryotic expression vectors cannot translateeukarytic sequences |
| | · eukaryotic genes have introns, and prokaryotesdon't |
| | · eukaryotic genes have exons and prokaryotesdon't |
I create a knockout mouseusing the agouti/black fur system \. When I cross the agoutioffspring of the originally engineered mouse, I find a ratio of 2agouti mice to 1 black furred mouse. What is the bestexplanation?
Answer not sure which one?!
| | · The gene knocked out was recessive. |
| | · The gene knocked out was dominant. |
| | · The gene knocked out was a lethalgene. |
| | · The knockput was integrated into a random spot,and did not knockput the original gene. |
A restriction enzyme cuts DNAand leaves the following end
xxCTGCA
xxG
Which of the following could be the sequence of the correspondingend of the other end of the cut DNA?
Answer
| | · xxG
xxCTGCA |
| | · xxC
xxGTGCA |
| | · xxCCGAT
xxG |
| | · xxGGCTA
xxC
An SNP always occurs dueto Answer
| | · a one base-pair change. | | | · a three base-pair change. | | | · a single restriction site change. | | | · a single amino acid change. | | | · None of these. | |
I PCR out a mutated gene in apatient with Lisenbee chorea (the inability to dance in acoordinated fashion) and I compare it to another patient with thatsame disease phenotype. One subject had a mutation on chromosome 4,and the other subject couldn't rock it because of a mutation onchromosome 5. This is an exampe of
Answer
| | · pleitropy |
| | · locus heterogeneity |
| | · allelic heterogeneity |
| | · dominant negative mutation |
| | · penetrance In his experiments, Mendelnoted that when two traits are involved in a genetic cross, theyare inherited independently of each other. Though Mendel didn'tknow about chromosomes, this still holds true (mostly)because Answer
| | · genes on the same chromosome are often mixedthrough meiotic crossover during the formation ofgametes. | | | · variant alleles of the same gene do not interferewith the phenotypic expression of the gentoype in different geneticloci. | | | · most higher organisms are diploid, making only twoalleles available for every genetic locus, and there is a 50/50chance of geting either allele from a single parent. | | | · expression of phenotypes can vary from thegenotypes because of encironmantal influences. | | | · chromosomes often recombine | Anticipation is caused by amutation that increases in expressivity over subsequentgenerations. Answer · True · False Question 44 I have a genotype that shouldproduce a specific phenotype, but some of the individuals with thegenotype do not demonstrate any evidence of the phenotype. I wouldconsider this an example of Answer | | · allelic heterogeneity. | | | · locus heterogeneity. | | | · penetrance. | | | · expressitivity. | | | · sheer luck. | Question Which of the following doesnot occur during the PCR reactions? Answer | | · Changes in the reaction temperature by athermocycler | | | · Denaturation at high temperatures | | | · Synthesis of oligonucleotide primers | | | · Extension of primers by DNA polymerase | | | · Annealing of oligonucleotide primers | Question DNA markers, or variantnon-coding regions of DNA, are DNA polymorphisms that are usefulfor genetic mapping. Answer · True · False
|
A mosaic is an organismwith
Answer
| | · multiple genotypes within one organism |
| | · multiple alleles within one genotype |
| | · more than one color of fur |
| | · transgenes added to the zygote beforedevelopement |
| | · a wt phenotype but a mutated genotype |
Question
Genotype causesphenotype.
Answer
| | · No, gentoype just influences phenotype. |
| | · Yes, genotype is the DNA sequence that createsphenotype. |
Question
A true genetic chimera can becreated by
Answer
| | · mutating a gene early on in the development of anorganism resulting in different alleles being present in theadult |
| | · multiple fertilized eggs or zygotes fusing to formone embryo |
| | · adding a transgene to the genome of an organismduring fetal development only |
| | · adding cells of a different species to an adultorganism |
| | · adding a transgene to the genome of an animal atany stage in development |
Question
The ABO blood group can bestbe explained by the concept of _______.
Answer
| | · dominant traits |
| | · recessive traits |
| | · allelic heterogeneity |
| | · locus heterogeneity |
| | · vampirism |
If a loss of functionmutation creates a dominant phenotype, it may be becauseof
Answer
| | · haploinsufficiency |
| | · penetrance |
| | · expressivity |
| | · allelic heterogeneity |
| | · locus heterogeneity |
Please select the best matchfor each.
Answer
| · PCR: Technique used to generatecopies of a DNA fragment | | · Southern blot : Screeningprocedure used to assay DNA fragments | | · RFLPs : DNA fragments generatedby restriction enzymes that vary in length betweenindividuals | | · Northern blot : Screeningprocedure used to assay mRNA fragments | | · Electrophoresis Movement ofmacromolecules in an electrical field | | Answer A. | Screening procedure used toassay DNA fragments | B. | Movement ofmacromolecules in an electrical field | C. | Screening procedure used toassay mRNA fragments | D. | DNA fragments generated byrestriction enzymes that vary in length betweenindividuals | E. | Technique used to generatecopies of a DNA fragment | |
A gene mutates, and theprotein produced has a novel way of interacting with the cell, andcreates a new phenotype because of this new functionality. This iscalled
Answer
| | · pleitropy |
| | · locus heterogeneity |
| | · allelic heterogeneity |
| | · dominant negative mutation |
| | · gain of function dominant mutation |
Question 62
Mutations in the somaticchromosomes may be inherited by the next generation.
Answer
· True
· False
A degenerate PCR primer withmany variant sequences must be used to make multiple copies of agene
Answer
| | · if only the protein sequence of the gene productis known to construct the primers |
| | · if the DNA you are using is cDNA to constructthe primers |
| | · if the DNA you are using is genomic DNA to becopied |
| | · if the DNA you are trying to copy iscDNA |
| | · if the vector is prokaryotic and the transformedcell is eukarytotic |
Question
Please select the best matchfor each term.
Answer
| · Restriction enzyme |
| · PCR C | | · Plasmid | | · YAC A | | · Dideoxynucleotide | | Answer A. | Endonuclease isolated frombacteria | B. | Eukaryotic cloningvector | C. | Method for amplification ofDNA sequences | D. | Small circular fragment ofDNA | E. | Molecule used in DNAsequencing | |
Question
A couple goes on MauryPovitch, and the results are in: you are not the father. But noother man impregnated the female (granted, unlikely for a MauryPovitch guest, but work with me here) and he must be the father.DNA analysis claims otherwise, though the child definitely wasmom's (poor thing). What may be going on here?
Answer
| | · the child is parthenogenic because the motheractually impregnated herself like a shark, and the child's DNA isall mom's |
| | · the child had a mutation that changed the genethat is used to trace paternity |
| | · the child is a mosaic because he is actually a setof twins fused early during fetal development, and therefore camefrom 2 eggs and 2 sperm |
| | · the dad may have germ-line mosaicism, meaning thatthe genotype of his sperm is different from his somaticgenotype |
| | · mitochondrial DNA only comes from mom, so there isno way to tell whobthe baby's father is |
