Biochemistry 2280A Study Guide - Quiz Guide: S Phase, Bromodeoxyuridine, Thymidine

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Mclachlin

Document Summary

Dna replication -> s phase of cell cycle: stages of dna replication: [interphase: g1 phase, s phase, g2 phase, m phase. Thymidine, 5"-bromo-2"deoxyuridine (brdu: s phase -> 5 patterns of dna replication. Depend on type of dna replicated: euchromatin 1st. Areas of active, uncondensed eukaryotic chromosomes -> s phase -> cell cycle: heterochromatin 2nd. Condensed areas of inactive chromatin -> dna not transcribed -> s phase -> cell cycle: helicases. >separating strands of dna helix: replication fork. Region of dna double helix -> strands separate -> synthesis -> daughter strands: template-dependent dna synthesis. Process -> daughter cells -> synthesised -> dna fork. Process of template-dependent dna synthesis daughter cells synthesised. > replication fork -> template = dna. Primer -> initiate synthesis: dna-dependent dna polymerase. Enzyme -> synthesises -> copy of dna -> dna template: primer. Main enzyme -> dna replication -> bacteria. Enzyme -> primes dna replication -> eukaryotes. Main enzyme -> primes dna replication -> eukaryotes.