Biology 2581B Study Guide - Midterm Guide: Dosage Compensation, Germline Mutation, Nondisjunction

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5 Oct 2015

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Marking: best answer b 1 mark (frequency); other marks for 3" sticky ends d 0. 5 mark; A 0. 5 mark: a linear double stranded dna molecule has two target sites for restriction enzyme ecoi. 1: you are given a sample of a linear double stranded dna fragment. You are to determine the number and order of restriction cut sites in that dna. In your first experiment you digest one aliquot of the dna sample with ecorv (rv) and after gel electrophoresis you observed three bands indicating fragments 30kb 15kb and 6kb in size. You then digest the second aliquot with ecori (ri) and after gel electrophoresis you observe four bands indicating fragments that are 18kb, 15kb, 10kb and 8kb in size. With the third aliquot you perform a double digest using ecorv and ecori and you observe four bands indicating fragment sizes of 15kb, 8kb 7kb and 3kb in size.

Related Questions

Recombinant DNA Technology

The questions all relate to the same topic and are somewhat interrelated

1. For gene cloning, a geneticist digests DNA with ___ an enzyme that cleaves DNA at sequence-specific sites.

A. DNA polymerase.

B. Ligase.

C. Restriction endonuclease.

D. cDNA.

2. Certain endonucleases cut DNA and leave DNA termini without overhangs which are called ___

A. Cohesive termini.

B. Sticky ends.

C. Blunt ends.

D. Oligonucleotides.

3. An enzyme found in retroviruses (like HIV) that can be used to prepare DNA fragments from mRNA is called ___.

A. Reverse transcriptase.

B. RNA polymerase.

C. DNA polymerase.

D. Integrase.

4. After combining DNA fragments in a cloning experiment, ___ is used to covalently to join the DNA segments.

A. Restriction endonuclease.

B. DNA ligase.

C. DNA polymerase.

D. Helicase.

a. Fred and Helenâs conceived child: Child ____

b. George and Helenâs conceived child: Child ____

c. George and Helenâs adopted child: Child ____

rosecrab387

We have four DNA sequences that we need to digest (cut up), and have 6 restriction enzymes in stock in our lab. We want to find out which restriction enzyme we can use on each sample.

For each sequence, please indicate which restriction enzyme(s) would cut within each sequence. Each sequence may be cut by one, more than one, or no restriction enzymes.

Following the sequences are two questions. Please choose from the same list of enzymes to indicate the correct answer to these questions.

Restriction enzyme	Digestion pattern
EcoRI	5' G\|AATTC 3' 3' CTTAA\|G 5'
HindIII	5' A\|AGCTT 3' 3' TTCGA\|A 5'
BamHI	5' G\|GATCC 3' 3' CCTAG\|G 5
AluI	5' AG\|CT 3' 3' TC\|GA 5'
SmaI	5' CCC\|GGG 3' 3' GGG\|CCC 5'
HbaI	5' GCG\|C 3' 3' C\|GCG 5'

(The | symbol indicates the specific cut sites.)

Each answer may be used once, more than once, or not at all.

1.	5' -TAGACTGAATTCAAGTCA- 3' 3' -ATCTGACTTAAGTTCAGT- 5'
2.	5' -ATACGCCCGGGTTCTAAA- 3' 3' -TATGCGGGCCCAAGATTT- 5'
3.	5' -CAGGATCGAAGCTTATGC- 3' 3' -GTCCTAGCTTCGAATACG- 5'
4.	5' -AATAGAATTCCGATCCGA- 3' 3' -TTATCTTAAGGCTAGGCT- 5'
5.	Which restriction enzyme(s) is(are) 4-cutters (i.e. they recognize and cut at 4-bp sequences, instead of 6-bp sequences)?
6.	Which restriction enzyme(s) do not leave a "sticky end" after cutting the DNA?

A.	Alu
B.	AluI, SmaI
C.	All 6 of these restriction enzymes.
D.	None of the 6 restriction enzymes listed.
E.	BamH1
F.	HindIII, AluI
G.	EcoR1
H.	SmaI
I.	SmaI and HbaI
J.	HbaI
K.	HindIII
L.	AluI, HbaI
M.	EcoRI, AluI
N.	AluI
O.	BamHI, AluI

sangriapug213

1.) A restriction recognition site is repeated 1 times on a circular DNA molecule. If you treat this DNA molecule with this restriction enzyme, how many DNA fragments will you get?

a. 1

b. 2

c. 3

d. Restriction enzyme does not cut a circular molecule

2.) In an agarose gel DNA electrophoresis, the DNA fragments are separated based on their

a. Size

b. Electrical charge

c. Nucleotides sequences

d. Density

e. All of the above

3.) DNA molecules are usually separated using agarose gel rather than polyacrylamide because:

a. Agarose polymerizes faster than acrylamide gel

b. Polyacrylamide gel has pore size larger than the size of DNA molecules

c. Agarose gel do not have pores on tis matrix and can hold large DNA molecules

d. Agarose gel has larger pore size similar to the size of DNA molecules

4.) Restriction endonucleases

a. Randomly recognize and cut DNA sequences

b. Hydrolysis the phosphodiester bond of DNA backbone

c. Recognize specific sequence on double stranded DNA

d. A and B

e. B and C

taupefrog573

Biology 2581B Study Guide - Midterm Guide: Dosage Compensation, Germline Mutation, Nondisjunction

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