Biology 2581B Study Guide - Final Guide: Immunology, Embryonic Stem Cell, Polytene Chromosome
10 May 2016
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Can be used to identify rare mutations in haploid cells & then combine them with mutations in diploid cells for complementation analysis. Large quantities of offspring can be easily produced. Can count the number of individual buds to determine the number of replications that it had undergone. Procedure transformation with plasmids, followed by recombination transgenetic markers. Drug resistance (amp-resistant, or leu2) advantage allow for observation of all properties / processes of eukaryotes. Ex: cell cycle, cell division, meiosis, secretory mechanisms, protein traf cking, chromosomal structure, signal transduction pathways, replication shuttle vectors disadvantage. Procedure knows: the number of divisions / pathways needed to specialize a particular cell, the exact location of genes. Easily tracked / observed mutations (since scientists already know the normal function of each cell / gene) During the rst mitotic division in the zygote, it will produce 2 daughter cells. Injected with dsdna maintained as extrachromosomal copy.
