BIOL 2070 Study Guide - Final Guide: Polyacrylamide Gel Electrophoresis, Polymerase Chain Reaction, Usp7
Document Summary
Volume ranges for micropipettes: 20 l micropipette: 2-20 l in 0. 02 l increments, 200 l micropipette: 20-200 l in 0. 2 l increments, 1000 l micropipette: 100-1,000 l in 2. 0 l increments. Imidiazole: disrupts the interaction between 6xhis in his-tag and ni2+ and releases his-tagged affinity protein. Usp7: ubiquitin specific protease that acts as a deubiquitylating enzyme meaning it removes ubiquitin from substrates. Usp7 stabilizes the substrate proteins and has an impact on p53 deubiquiylates p53 and protects it from degradation (tumor suppressor function) Epstein-barr virus (ebv): human herpes virus 4 (hhv-4), is a virus of the herpes family associated with particular forms of cancer, such as hodgkin"s lymphoma, burkitt"s lymphoma, nasopharyngeal carcinoma, and central nervous system lymphoma associated with hiv. Epstein-barr nuclear antigen 1 (ebna1): multifunctional viral protein associated with the ebv virus. Ebna competes with p53 for binding usp7 and prevents usp7 from binding to p53 and stabilizing it.
