BIO 100 Quiz: ap10_biology_form_b_q1.pdf
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Biological molecules can be separated by using chromatographic techniques. The diagram shows the separation of several spinach leaf pigments by paper chromatography. Using the diagram, (a) explain how paper chromatography can be used to separate pigments based on their chemical and physical properties. (4 points maximum) Chemical similarity between solvent/pigment (solvent: pigment) further movement. Show your calculations. (2 points maximum: formula or description dpigment/dsolvent, calculation 3. 5/7. 5 0. 5. A total of 4 points were earned in part (a) for explaining two factors that affect pigment migration during paper chromatography. The first 2 points were earned for the explanation that strong interactions between the paper and the pigment will retard the pigment"s movement. The second 2 points were earned for indicating that pigments that dissolve better in the solvent will diffuse further than those that do not dissolve as readily. The molecular size points were not awarded since the maximum 4 points had already been earned.
