PLANTBI C112 Midterm: 2 Microbial Growth
23 Dec 2018
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When bacteria have enough of all required nutrients to duplicate all of their structures and contents and divide. Rich/complex : not chemically de ned, complex organic molecules, wide variety of nutrients supplied by peptone + organic compounds to break down. De ned/minimal : precise amount of known chemicals, may/may not contain organic compounds. Cells need: water, c, n, p, s, k, mg, ca, fe, and traces of se, mn, co, zn, cu (and. Total cell yield : determined by the most limiting nutrient. Batch culture: closed system where bacterial growth depletes nutrients and alters environment. Occurs when stationary phase is diluted to fresh medium or when cells are transferred from rich to minimal medium. Bacteria have to sense new environment or synthesize either: Many proteins needed for rapid growth that weren"t produced during starvation. Speci c proteins needed to produce the nutrients not already in medium.
