PCB 3134- Midterm Exam Guide - Comprehensive Notes for the exam ( 24 pages long!)
Document Summary
Cytoskeleton: network of interconnected filament and tubules extending throughout cytosol. *immunofluorescence microscopy: can stain the actin filaments for example. *inhibition of mt formation by colchicine or nocodazole. Taxol cmpd extracted from bark of pacific yew tree required lot of bark to make little sample. Structure: 3 aromatic rings, amide, ester, alcohol, alkene, ketones. Incorporates into the -tubulin subunit and changes dynamics of its gtpase activity. *tension of mt changes when the gtp of the dimers hydrolyzed into gdp + pi so disassembly now easier, but taxol inhibits this. Hollow tube w/ wall made of 13 protofilaments (subunit) consisting of and monomers combined to form -tubulin heterodimers. Outer diameter, 25nm, greater than inner diameter, 15nm, obviously. Has (+) (where the monomers are) and (-) (where ends w/ monomers) ends (polarity) Found as singlets, doublets, or triplets in cells. *organization and maintenance of animal cell shape and polarity. *each is a tubulin subunit of the dimer.
