BIOS-115 Study Guide - Midterm Guide: Prokaryotic Small Ribosomal Subunit, Open Reading Frame, Kozak Consensus Sequence
12 Feb 2018
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Peptide bond: bond formed between amino acids; dehydration synthesis reaction; Basic structure of amino acid: amino group (nh3 side chain. Eukaryotic eif-4f protein complex: eif-4e cap binding protein and eif-4g binds. No shine-dalgarno sequence 40s subunit + trna + eif"s + gtp scans mrna for kozak sequence. 60s subunit binds, eif"s dissociate except eif-4f 80s complex. Elongation trna + ef-tu-gtp binds to a site. Peptide bond forms via peptidyl transferase activity from large subunit. Ribosome recycling factor (rrf) binds to a site. Rrf, ribosome disassembles eef-1a (replaces ef-tu) eef-1b (replaces ef-ts) eef-2 (replaces ef-g) erf1 (replaces rf1) erf3 (replaces rf3) eef-2 (replaces ef-g) Prokaryotic ribosome: 50s and 30s subunits; 50s = 23s rrna + 5s rrna; 30s = 16s rrna; creates 70s complex. Eukaryotic ribosome: 60s and 40s subunits; 60s = 28s rrna + 58s rrna; 40s = 18s rrna; creates 80s complex. Somatic mutation: mutation that affects only the individual and is not heritable.
