BIOL-UA 21 Study Guide - Midterm Guide: Deoxyribonuclease, Chromosome, Repressor
15 May 2016
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Dnase i footprinting only see fragment that has label on end. If labeled everywhere, see all fragments from digest smear and no footprint because everything would be there. Bp distances differ along gel non-linear relationship between distance size. Something smaller moves way faster, therefore it"s easier to see gaps at the bottom of the gel. Most dna is not cut at all (largest band is the 220 bp) Polynucleotide kinases label end (labels both ends and then have to cut fragment) Kinase exchanges phosphate group at 5" dna strand. Stock = family of flies that have same sets of chromosomes. Balancer chromosome with 2 copies is homozygous lethal. Jlo x cyo = jammed and curly because both dominant cross again, and if jammed is homozygous lethal, never see any straight winged flies. Avoid recombination multiple inversions and no recombination. Test to see whether/not jammed can balance another mutation on second chromosome. Hallmarks of balancer: dominant, homozygous lethal, avoid recombination.
