[BIL 250] - Final Exam Guide - Everything you need to know! (24 pages long)

• School: University of Miami
• Department: Biology
• Course: BIL 250
• Professor: Kevin Mc Cracken

Document Summary

To kill the mice: bacteria had to be alive and have the polysaccharide coat. Incorrectly believed that protein was the transforming agent. Iiis dna (cid:449)as the ge(cid:374)eti(cid:272) (cid:373)aterial i(cid:374) griffith"s e(cid:454)peri(cid:373)e(cid:374)ts (cid:894)(cid:374)ot rna(cid:895) **deciphered dna, not protein was the genetic material that transformed in. Main features of double helix model of dna. Sugar-phosphate backbones on the outside, bases towards central. Sugar-phosphate backbones not equally spaced = different sized grooves. Phosphodiester bond: covalent bond between the phosphate group of o(cid:374) (cid:1009)" (cid:272)ar(cid:271)o(cid:374) o(cid:374) o(cid:374)e (cid:374)u(cid:272)leotide a(cid:374)d the (cid:1007)" (cid:272)ar(cid:271)o(cid:374) of sugar. Strong bonds -> reason for dna being hard to degrade (autoclave) Supercoiling: 2x helix twisted in about its own axis controlled by topoisomerases (enzymes) Histones: abundant, basic proteins with (+) charge that bind to dna. Nonhistones: other proteins with dna with (-) charge. Connected by linker dna (beads on a string) Specialized sequences function with microtubules and spindle apparatus (mitosis/meiosis)