ART 9C Study Guide - Quiz Guide: Green Fluorescent Protein, Halorhodopsin, Optogenetics
Document Summary
Part i understanding optogenetics: during week 5, we will use optogenetics to study the neuronal populations that are involved in controlling habituation. Pmec-4::chr2 is a worm that expresses chr2 under the pmec-4 promoter. This means that chr2 is expressed in any cell that pmec-4 is expressed. In other words, chr2 is targeted to the pmec-4-expressing neuronal population. Tph-1::archt::gfp is a worm that expresses archt under the tph-1 promoter. Archt is tagged with green fluorescent protein (gfp). Like halorhodopsin, archt is an optogenetic tool that silences whatever neuron in which it is expressed. In contrast to halorhodopsin which allows chloride anions to pass into the cell, thereby hyperpolarzing it, archt is a proton pump which hyperpolarizes a cell by pumping protons out. Myo-3::halo/nphr::cfp is a worm that expresses halo/nphr under the myo-3 promoter. Halo/nphr is tagged with cyan fluorescent protein (cfp). What kind of worm is tph-1::chr2::gfp: the key to implementing optogenetics is where your optogenetic tool (chr2,
