BIO SCI N113L Study Guide - Quiz Guide: Allen Brain Atlas, Polyclonal Antibodies, Monoclonal Antibody
Document Summary
Distinguish research questions which can be answered by immunohistochemistry. Interpret hypothetical immunostaining to determine whether a primary antibody was specific to an antigen. Monoclonal antibodies are specific because collected from a single immune cell. Polyclonal antibodies come from different immune cells that may recognize different parts of the injected antigen. If there is colocalization with another protein, that means the protein is expressed in the nucleus. List applications in which reference to the allen brain atlas may be useful. Anatomical areas a gene/protein target is expressed. Can find regions that express similar genes. Describe how adjustments of the pinhole will affect confocal microscopy resolution. Detection removes all emission not originating from focal plane: filters depth of focus, spatial filter. If increased, more extrafocal light will reach and will fog the sharp information b/c unwanted blurry signal (bad spatial resolution) Describe trends when fluorophore excitation and emission wavelengths are compared. Excitation is around 100nm lower than emission spectra.
