MIMG 101 Study Guide - Midterm Guide: Axoneme, Basal Body, Microsporidia

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 101
• Professor: G U N S A L U S& B R A D L E Y

Document Summary

Obligate intracellular must live within human cell: toxoplasma. Dangerous for immunocompromised patients: polar tube structure -> harpoon cell than transfer microsporan inside. Polymerization of tubulin at the + end -> oligomers -> microtubules. 9+2 axoneme arrangement of microtubules: dyneins connect outer mt pairs, radial spokes connect outer pairs to central pair. Basal body in cytoplasm (vs in the membranes: made of centrioles, flagella extend from basal body but form axoneme with central pair. Flagellar motility test: observe isolated flagella beats on own, test in vitro, add detergent to remove plasma membrane, add protease to digest cross links, add atp, buffer. Outer pairs slide apart and are thus atp dependent. Flagellar motion test: isolate, add protease, fix to solid support, add atp, buffer. Observe whip like beating instead of sliding due to dynein. Mutants may lack dynein, spokes, or central pair. Plasma membrane + inner membrane complex (with myosin a) + gac bridge: micronomes within.