BIOL 1107 Study Guide - Midterm Guide: Phosphodiester Bond, Okazaki Fragments, Exergonic Reaction
Document Summary
Secondary hydrogen bonding of the base pairs. Highly ordered shape of dna allows for enzymes to easily see if something is wrong in the morphy. The old strand is cut up and interspersed with the new one. Ssbp provide stabilization of the open nucleotides. Everything continues down from the replication bubble. Primase is the landing site for dna polymerase. Provides a free 3" -oh group for synthesis of the phosphodiester bond. Both the leading and lagging strand use the same system to replicate. Looking over of the dna nucleotides to ensure proper binding - dna polymerase. Connection to the okazaki fragments - ligase. Dna polymerase catalyzes a condensation reaction with deoxyribonucleoside triphosphates (dntp) The cleavage of the phosphate groups is an exergonic reaction. Adds to the 3" end of the primer, working in the 5" to 3". A single stranded dna binding proteins (ssbp) stabilize single strands. Sliding clamp holds dna polymerase in place.
