BSC 2010 Study Guide - Spring 2018, Comprehensive Midterm Notes - Cell Membrane, Dna, Acid

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Lecture (cid:884) (cid:523)chapter (cid:883)(cid:887). (cid:884) up to (cid:498)gene flow may change allele frequencies(cid:499); Be able to recognize (or come up with) examples of each: positive directional selection, negative directional selection, stabilizing selection, disruptive selection, discuss the role of randomness in evolution. Add boxes and words to illustrate the role of glycosilation. If colonies are resistant, then the plasmid was taken up into the host cell: lacz: identifies if recombinant dna was inserted successfully, blue color: lacz gene not disrupted transformation successful but recombinant. Dna not inserted: blue color comes from production of the enzyme ( -galactosidase) coded by lacz, white (no) color: lacz gene disrupted transformation successful and recombinant. Dna was inserted: used as marker to show whether recombinant dna was inserted into plasmid. If colonies don"t produce b-galactosidase (produces white color), then lacz"s function was interrupted by insertion of foreign/recombinant dna.

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