BIOL 331 Study Guide - Midterm Guide: Lytic Cycle, Lac Repressor, Cyclic Adenosine Monophosphate
Document Summary
Principle of formation- to remove the cell wall of bacteria so that it is more permeable to chemicals and other substances. Hemacytometer and its uses: used to easily estimate the average number of spheroplast cells in the test and control group (pick 5 blocks) Function of sds- kills the spheroplast cells because they do not have a cell wall to protect them from the chemical. Function of lyticase enzyme- lyticase enzymes are the ones that destroys the ridged cell walls so that they are permeable to chemicals. Phage titer- nonliving, have no cell structure and cannot replicate by themselves. Virulent phage- can only undergo lytic cycle, always kills host cell: ex: t-phages, lytic life cycle: Attach to host- uses tail piece to attach. Injection of phage dna (contraction of tail sheath) Takes over host transcription machinery (sigma factor) to synthesize necessary components for assembling of new phages.