Biology And Biomedical Sciences BIOL 2960 Study Guide - Midterm Guide: Dna Polymerase Iii Holoenzyme, Rna Polymerase Iii, Dna Polymerase I
6 Feb 2019
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Synthesis of new strands: dna polymerase, requires ssdna template, dsdna must be unzipped by helicase, or heated (in vitro, requires a primer to donate a 3"-oh group, adds nucleotides 5" to 3". 3"-oh group: must use rna primers in vitro since they don"t require another primer to synthesize, helicase, separates dsdna strands, requires atp, single-stranded binding protein (ssbp, covers/protects exposed ssdna, holds strands apart. Most somatic cells lack telomerase, causing telomeres to shorten as the body ages (humans: possibly contributes to aging process, telomere length-aging hypothesis- simplistic, telomeres largely discounted as contributing significantly to aging, telomeres and cancer. Requirements for pcr: template dna, dna polymerase, dntp"s, buffer, two oligonucleotide primers (one per strand, usually 20-25 nucleotides long, usually dna primers used in vitro (chemically synthesized) Use of pcr for dna sample comparison: amplification of variable number tandem repeats (vntrs) and determine size of amplification product.
