Biology And Biomedical Sciences BIOL 2960 Study Guide - Midterm Guide: Polymerase Chain Reaction, Taq Polymerase, Dna Mismatch Repair
6 Feb 2019
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Study guide 9: pcr, dna sequencing, and dna repair (lectures 21-23) At the end of this unit, you should be able to define and/or use the following terms and concepts: Genetic terminology: mutation, mutant, types of mutations: base substitution, deletion, insertion. Dna repair: causes of dna damage and errors, dna replication: dna strand slippage, insertions, deletions, base substitutions, mispairing, dna damage: Techniques based on in vitro dna replication: polymerase chain reaction (pcr, template dna, taq dna polymerase, dntps (deoxynucleotide triphosphates), oligonucleotide primers ( oligos , denaturing, annealing, and polymerization steps; cycles; exponential amplification; thermocycler, dna sequencing, sanger dye-terminator sequencing: Dideoxy nucleotides (a. k. a. , 2",3"- dideoxyribonucleotide or ddntps) with attached fluorescent dye, template dna, oligonucleotide primers: next generation sequencing: massively parallel sequencing and direct detection of dna base incorporation during replication. When you"re done studying for this unit, you should be able to: explain the origin of dna errors or damage and the mechanisms that occur to prevent them from persisting.
