MOLBIOL 3B03 Chapter Notes - Chapter 6: P53, Neural Development, Ribonuclease
9 Sep 2017
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Recruits risc (rna induced silencing complex) and mirna + sirna to silence a transcript via cleavage or degrade the transcript before translation. Petunia studied by jorgensena: added an extra copy of chalcone synthase gene to create darker flowers, but observed the repression of the phemotype. White sections: rnai silenced the gene sirna degrade mrna. Source: exogenous form of rnai (ie. transposons, viruses), that integrate into human genome. Mutations are lethal and recessively inherited in diploid organisms: can use antibody neutralization and pharmacological perturbation, but that requires preparation and characterization of the target transcript and inhibitor. Studied unc-2: involved in muscle formation and maintaining shape of the worm, and its movement: when they injected sense and antisense rna separately, they got wt, when they injected both mixed => dsrna = 100% twitch. Can identify the physiological function of a gene- knock them down and study the effects. C. elegans: rnai feeding; mammalian cells transfection or lentiviral infection: via shrna.
