BIO130H1 Chapter Notes - Chapter 3: Endonuclease, Blast, Restriction Digest
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BIO130H1 Full Course Notes
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Dna sequencing precise order of nucleotides in a piece of dna is determined. Assuming both sets of plant materials are from the endangered orchid species . Task is to tell the diference between the wild and the genetically engineered. Paphiopedilum spicerianum orchid using pcr test will give info needed to report to supervisor. Comparative analysis are useful allow to infer structural, functional and evolutionary relationships. Comprised of a collection of all publicly available nucleotide and protein sequences. Blast score can measure if two sequences are homologous (share common. Single sequence that is compared against all other known sequences one-against-all evolutionary history); the more nucleotides that match, the higher score given. P=probability of obtaining an alignment with the score in question or better. P=1-e^-e (e value of calculated by p value) Multiple sequence alignments: msa used with these tools clustalw, clistalw2,
