GEN-3000 Chapter Notes - Chapter 12: Euchromatin, Consensus Sequence, Tata Box
Document Summary
Transcription: rna molecules are synthesized on a dna template. The amount of rna is proportional to the amount of protein in a cell. Transcriptional unit: stretch of dna that codes for rna + sequences necessary for transcription. Indicate which strand of dna will be transcribed, determine start site. Pribnow box = 10 nucleotides upstream of the initial site of transcription. Transcription is established when: rna polymerase sigma factor recognizes promoter. Enzyme explores dna strand till it finds promoter. Note: promoter recognition and binding is key to determine the frequency at which a gene is transcribed. Consensus sequence: present in a particular gene or protein. Pribnow box: consensus sequence located 10 nucleotides upstream of initial transcription. Mutations in regions could lead to diminished transcription. Cis acting elements: molecules that are adjacent parts of same dna. Cis acting elements: molecules that are adjacent parts of same dna molecule. Rna polymerase reads dna on the template strand.
