BIOLOGY 201L Chapter Notes - Chapter 8: Lac Operon, Lac Repressor, Regulatory Sequence

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14 Dec 2016
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Cell differentiation is achieved by changes in gene expression. Gene expression is a complex process by which cells selectively direct the synthesis of proteins and. Dna in specialized cell types of multicellular organisms still have entire set of instructions needed to form a whole organism. Mass spectrometry can analyze total protein content in a cell. Housekeeping proteins are proteins that are common to all cells of an organism. Include structural proteins of chromosomes, rna polymerases, dna repair enzymes, ribosomal proteins, enzymes involved in glycolysis and other metabolic processes, and proteins that form the cytoskeleton. Gene expression can be studied by cataloging cell"s rnas. Typical differentiated human cell expresses 5000-15000 protein coding genes from about 21000. Cell can change gene expression in response to external signals. Multiple rna polymerases can be transcribing at same time which increases gene expression and rate. Promotor sequence of gene determines rate at which gene is transcribed.

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