BIOL 2051 Chapter : Chapter7 Outline

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azuremammoth471

15 Mar 2019

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Sequence of nucleotides in dna that codes for a protein. _nucleotide_ sequence corresponds to sequence of _amino acids (what proteins are made of)_ Central dogma of molecular biology: basic processes are the same in prokaryotes & eukaryotes, but the organization of genetic information is more complex in eukaryotes, major differences: Eukaryotes (complicated; replication and transcription occur in nucleus, translation occurs in the cytoplasm) _introns_- dna sequences within genes that are not part of coding sequence for protein. Contain huge amount of noncoding dna between genes (never translated into gene) In humans over 90% of the total dna is noncoding (enhancer sequences, promoters, gene remnants, pieces of viral genomes like lysogenic phages) Chromosomes are usually circular (1 singular circular chromosome) Have little noncoding dna (less than 15%) Cluster of genes controlled by 1 regulatory sequence or promoter. Adenine pairs with thymine by 2 hydrogen bonds (a-t) - Guanine pairs with cytosine by 3 hydrogen bonds (g-c) -

Related Questions

What is DNA?

1. DNA is often referred to as a ladder. What pieces of the DNA molecule make up the rungs of the ladder (the steps)?

2. What pieces of the DNA molecule make up the sides of the ladder?

3. What types of bond occurs between the bases in the DNA molecule?

4. What makes up chromosomes? What is Gene?

5. How long does it take 1 cell to copy its genetic information? 6. How does the DNA copy itself so fast? What tricks does it use?

7. Describe how the bonds between base pairs work to hold the DNA strand together

DNA Replication

1. How many genes are in the human genome?

2. What are the differences between mRNA and DNA?

3. Where in the cell does transcription and translation occur? (give both places)

4. Which RNA piece reads the mRNA and how does it do this?

5. What is the start sequence? What amino acid does this code for?

6. What 3 codes tell ribosomal RNA to stop reading and release the protein?

DNA Extraction

1. List three differences between the virtual DNA extraction lab and the banana extraction lab you did last week.

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Biochem:

1. Indicate whether the following describes DNA polymerase, RNApolymerase, both, or none:

a. Binds to single stranded DNA

b. Uses an RNA primer

c. Has proof-reading ability

d. Reads from 3' to 5'

e. Phosphodiester bonds are formed using rNTPs

f. Requires a DNA primer

g. Multiple types are present in eukaryotes

h. Mutiple types are present in prokaryotes

i. Is synthesized bi-directionally

j. Requires there to be a terminal sequence on DNA

k. Is part of a larger complex made up of several proteins

2. Post-translational damage causes the loss of the 2' hydoxylgroup on several adenosine nucleotides within the intron sequenceof a primary RNA transcript. What could possible happen to theresulting mature RNA sequence and consequent protein?

3. Where on the below strand of RNA would you most likely findthe following:

a. Start of transcription

b. Start of translation

c. TATA box

d. Enhancers

e. Initiator

f. Activators

g. Repressors

4. How would the following mutation in the template strand ofDNA affect the translated protein? (x=any base, / = exon/intronjunction)

5-XXXXC/CTGAAGAGGAAGXXXXGTTAGXXXXACTCAC/CTCGC-3 to5-CCTGAAGAGGAAXXXXXXXXGTTAGXXXXACTCACCTCGC-3

5. When will there be a 5'-5' linkage in RNA? When is this bondformed? Which base is commonly involved in this process? What wouldhappen if there was an inability in this enzyme to make the 5'-5'likage?

6. Consider the following strand of mRNA. What protein would beproduced if this strand was translated?

5'- CGUAUGCCCUUUACUAGUAUGUGAUACUAGUAAACC-3'

7. What would be the new protein if the following mutations weremade to the strand in 6? Label/classify each type of mutation.

a. 5'-CGUAUGCCCUUUAACUAGUAUGUGAUACUAGUUAACC-3'

b. 5'-CGUAUGCCCUUCACUAGUAUGUGAUACUAGUUAACC-3'

c. 5'- CGUAUGCCCUUUACUAGUAUUGAUACUAGUUAACC-3'

d. 5'- CGUAUGCCCUUGACUAGUAUGUGAUACUAGUAAACC-3'

e. 5'-CGUAUGCCCUUCACUAGUAUGAGUAACUAGUAACC-3'

f. 5'-CGUAUGCCCUAAACUAGUAUGUGAUACUAGUAAACC-3'

8. What are examples of two different types of point mutationsin sequences coding for hemoglobin production?