BIOL 4246 Chapter : 6 7 12DNA Replication
Document Summary
Does so because you need to counter positive superhelical twists created by dna separation, gyrase"s negative superhelical twists cancel them out. Each domain has its own superhelicity state depending on the expression going on. Dna synthesis- you need an existing polymer with a free 3" end with an oh involved in nucleophilic acid of the alpha phosphate of the triphosphate to form a diester bond. The template dictates which nucleotide can stably bind to the site. The major dna synthesizing structure in bacteria is dna poly iii. Leads to processivity- once you start a process you keep going until the end. 5" to 3", this is 3" to 5" opposite of polymerase activity. Removes unpaired bases on the end of the strand. Using proofreading the misincorporation rate drops to 10e -7 or 10 e -8. 100-fold decrease in misincorporation: with this lower misincorporation rate, you will have 1 out of every 2 cells mutant.