CHEM 2001 Chapter : Problem Set 17KEY
Document Summary
In substrate-level phosphorylation, a phosphate group is transferred to adp from another molecule with high phosphoryl transfer potential. This reaction is done by a specific enzyme using a specific substrate. In oxidative phosphorylation electron transfer is used to generate a proton gradient across the inner mitochondrial membrane. The energy from the proton gradient is used by atp synthase to generate atp from adp and pi: describe the catalytic mechanism of atp synthesis by the f1 portion of atp synthase. The beta subunit is the catalytic subunit for atp synthesis. Each beta subunit must go through three different conformations (o l t) in order to complete the reaction (substrate binding, catalysis and product release). These conformational changes are induced by the beta subunits" interactions with the central gamma subunit. Three beta subunits interact with the central gamma subunit, each having a distinct conformation.
