BIO SCI E154 Chapter 7-8: BIO SCI E154 Chapter 6-8: Chapter Summary 4
18 Dec 2016
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Mutations are more likely to harm a protein"s function than to change it. Therefore, a new mutation most likely will be a loss of function than a gain of function. *remember loss of function reduces function, gain of function can make new function. Can conclude that loss of function mutations are usually recessive, so new mutations are recessive. Heterozygotes are carriers - don"t have disease but have cellular phenotype some rbc exhibit sickling. Separate large molecules (proteinsp in electric field, based on size, charge, shape. Single point mutation --> single amino acid change -> portein causes long aggregates in low o2 --> cause sickling --> no oxygen to parts of body --> anemia causes blood cells to die. Molecular probes - antibodies/single sranded nucleic acids --> bind to specfic target molecules to detect protein or dna/rna. Transfer of nulciec acids or proteins from gel to membrane. Southern blotting - transfer of dna from gel to membrane, followed by molecular probing.
