MCD BIO CM156 Chapter Notes - Chapter N/A: X-Inactivation, Xist (Gene), Human Genetics
23 Feb 2020
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Xist/xist rna clouds: despite having strong specific immune responses, females are more likely to develop autoimmune disorders than men will because of their weaker immune system. No breakdown of self-tolerance, and they produce more serum igm and antibodies, but can"t protect themselves against specific diseases: single cell rna fish analysis: In vitro activation: of lymphocytes and t cells. Used cd3/cd28 and performed a time course analysis of xist rna fish: x-linked immunity genes cd40lg and cxcr3 are biallelically expressed in some cells. Xist rna clouds returne to the xi 24 h after in vitro stimulation. Cot-1 rna detects regions of nascent transcription within the nucleus. H3k27me3, h4k20me, macroh2a, and h2a-ub on the xi over-express a variable set of x-linked genes. Cd40lg and cxcr3 expression in human female t cells: inactive x"s might become partially reactivated in female lymphocytes. Xist/xist rna binding proteins are critical for recruitment of xist/ xist rna back to the xi.
