MCDB 351 Chapter Notes - Chapter 12-16: Flp-Frt Recombination, Green Fluorescent Protein, Transgene

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 351
• Professor: Richard Hume

Document Summary

Tabtila: #2 a, c, 3b, 4a: the yeast flp recombinase recognizes frt sites in dna and does a reaction analogous to cre acting at loxp sites (removes sequence between the sites). Frt sites have a completely different sequence than loxp sites, so this system can be used in combination with cre-lox in mammals or. You build lines of fruit flies each with one transgene, and then cross the flies so that all three transgenes are in a single line of progeny. If all transgenes are active in cells, these would be the only cells that would glow green due to successful expression of egfp, since stop region is removed. Gal4, which acts on uas, thus activating the target sequence: frt-stop-egfp-frt. We also understand that neuropeptide z promoter drives flp and flp recombinase recognizes frt sites in dna and removes sequences between the two frt sites.