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Introduction to Molecular Biology

Exam Study Guides for LIFESCI 3

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LIFESCI 3 Study Guide - Final Guide: Allolactose, Inverse Relation, Signal Transduction

1) Eukaryotic Transcription a) 3 different RNA polymerases used i) RNA pol. I: pre rRNA (1) Encodes rRNA responsible for protein synthesis in the ribosome ...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Study Guide - Midterm Guide: Polyacrylamide Gel Electrophoresis, Northern Blot, Hydrophile

Central Dogma: DNA > translation > RNA > transcription > amino acids/proteins 1) DNA a) Discovery i) Griffith experiment: identified "transforming principle" that was making heat-kill...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Study Guide - Midterm Guide: Euchromatin, Interphase, Endonuclease

1) DNA arrangement in the cell a) DNA is compacted into chromosomes i) Compacts DNA so it can fit in the cell ii) Protects DNA iii) Regulates accessibility proteins have to DNA iv) Allows efficient transmission of DNA to d...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3- Final Exam Guide - Comprehensive Notes for the exam ( 158 pages long!)
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[LIFESCI 3] Comprehensive winter guide including any lecture notes, textbook notes and exam guides.find more resources at oneclass.com LS 3 - Lecture 1 - Syllabus & intro to DNA General Info âÃ...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3- Final Exam Guide - Comprehensive Notes for the exam ( 28 pages long!)

[LIFESCI 3] Comprehensive winter guide including any lecture notes, textbook notes and exam guides.find more resources at oneclass.com LS 3 - Lecture #3 - DNA Experiments & Proteins Important Experiments Related to D...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 26: Sticky And Blunt Ends, Drosha, Allosteric Regulation
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Lecture 26 recap gal Multiple layers of signaling Default state: gal 4 and gal 80 expressed constitutively - Gal 4 binds upstream activation sequences for Gal1 gene; activation domain i...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 25: Nucleosome, Corepressor, Transcription Factor
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Lecture 25 Tryptophan recap Operon makes genes of enzymes that make tryptophan (biosynthetic pathway) Tryptophan regulated by tryptophan binding tryptophan repressor -> conformation...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture 28: Lecture 28 cloning II
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Lecture 28 insulin E.g. ampicillin + unregulated Lac Z or dual antibiotics Grow selected bacteria on agarliquid culture Isolate some plasmid DNA digest (length of plasmid vs insert) Affinity chromatography Cloning applica...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture 27: Lecture 27 cloning I
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Lecture 27 Cloning Extrachromosomal DNA gets inside cell > expressed in addition to natural genome Vectors (= plasmid for the sake of the class) Extracellular DNA > utilizes host machinery transcribe information from it E...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 21: Prokaryotic Small Ribosomal Subunit, Consensus Sequence, Ribozyme
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Lecture 21 Wobbly base pairing continued Happens at most 5 position of anticodon (on tRNA), complementary to most 3 end of mRNA codon (translation happens 5 3) Special base on tRNA at this region Accounts for discrepancy ...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 24: Flac, Dna-Binding Domain, Tryptophan
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Lecture 24 Lac Z encodes BetaGal Lac I encodes Lac repressor Lac P is Lac Operon promoter (promoter region for Z, Y, A genes) O is operator: repressor binding site located in promoter region > prevents RNA pol. from bindin...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 22: Release Factor, Electrophile, Ribosome
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Lecture 22 Recap EK translation Kozak sequence contains AUG; spans from 5 UTR to coding region (in PK cell Shine Dalgarno sequence completely outside of coding region) UNIQUE to EK cell: cyclical translation (Translation ...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 23: Wild Type, Allolactose, Flac
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Lecture 23 Antibiotics Bacteria ribosomes are common target of antibiotics Antibiotic e.g. Puromycin Puromycin fits in A site of ribosome when catalytic RNA (23S rRNA 16S rRNA) try to catalyze peptide bonds between amino ...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 20: Base Pair, Leucine, Wobble Base Pair
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Lecture 20 A charged tRNA has an aa attached to it Modification on tRNA: connect aa to it There are several tRNAs for each aa Anticodon on tRNA match codon on mRNA for an aa (decoding via tRNA) aminoacyltRNAsynthetase (e...

Life Sciences
LIFESCI 3
Amy Fluitt
LIFESCI 3 Lecture Notes - Lecture 16: Helicase, Exonuclease, Phosphodiester Bond
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Lecture 16 EK cell transcription Similarities between prokaryotic and eukaryotic transcription Both synthesize RNA from 5 to 3 Transcription initiates at a promoter Both utilize RNA polymerases that share similar structure...

Life Sciences
LIFESCI 3
Amy Fluitt

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