MIIM20001 Lecture 19: Lecture 19 – Microbial Genetics
Document Summary
Polynucleotide sequence that codes for a functional product. Bacterial gene structure: genes highly organised, diagram: double stranded dna on top of mrna copy on bottom, dna gene includes specific elements, region: leader sequence, terminator sequence- important for translation. Promoter: rna polymerase recognition/binding site, upstream of coding region, variation in sequence (allows for variation in expression, two regions, one at -35 and another at -10. Rna polymerase recognition binding site, occurs upstream of the coding region. Promoters vary between genes and bacterial species. Most important regions -35 and -10, occur 10 and 35 nucleotides before a in the aug start codon +1 nucleotide. Not a sequence that is transcribed signal to begin transcription. Involved in initiation of translation and may be involved in regulation of transcription or translation (binding site for regulatory proteins) Leader sequence portion of the transcript where ribosomes can bind in order to start translation. Terminators and trailers: transcription continues beyond coding region, terminator found in trailer region.