PHYS30005 Lecture Notes - Lecture 1: Myocyte, Percutaneous, Myosin
13 Aug 2020
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Document Summary
Human muscle tissue sampling using the percutaneous needle biopsy technique. Can see slow twitch fibres: alkali: fast twitch fibres stain darkly, immunoblotting. Type i fibres more dependent on oxidative energy system. Type ii fibres tend to be bigger. Increase activity requirements -> increase recruitment of slow twitch fibres + increase recruitment of iia fibres initially. Reaching maximal levels -> recruit the iib fibres". First row of numbers: change in glycogen within individual fibre types. 2nd row of numbers: # of fibres that were counted. Low -> medium -> high start to rely more on greater proportion of fast twitch fibres. Endurance athletes might have more slow twitch fibres. Sprinters might have more fast twitch fibres. Cross section lower leg muscle of marathon runner: (preincubated in acid so slow twitch fibres stain dark: predominantly slow twitch. Looked at slow twitch fibre in leg vs. arm. Runners had relatively greater numbers of slow twitch in legs.
