BABS1201 Lecture 13: BABS1201 LEC 12,13
BABS1201 Notes – LEC 12, 13
LEC 12: Continued…
• Light independent:
❖ RuBisCO is an enzyme that catalyses the carbon fixation to RuBP.
❖ RuBP is the CO2 acceptor in the first major step of carbon fixation.
• Calvin cycle:
LEC 13: DNA Replication
1. DNA strands are separated by the helicase to form a replication fork
2. Nucleotides are added to the two parental strands to form the sugar phosphate
backbone
a. Leading strand – DNA polymerase binds to primer and starts replicating DNA
with no breaks in 5’ to 3’ direction
b. Lagging strand – formed in Okazaki fragments. DNA polymerase is attached
to primer and adds a short row of bases. Exonuclease removes all RNA
primers (from both strands). A new DNA polymerase adds bases in empty
spots to fill the second daughter DNA strand.
3. The separate daughter strands are sealed by DNA ligase
• Multiple origins of replication are required for efficiency
• DNA strands run antiparallel
RuBP + C
Split in half
Phosphorylation