BIOL2202 Lecture Notes - Lecture 9: Joshua Lederberg, Relaxase, Dna Replication
Document Summary
During conjugation: the donor plasmid is replicated, one strand of the double stranded dna is being fed in through the trag channel and into the recipient cell, this method is called the rolling cycle replication. Rolling cycle replication: dna replication is happening simultaneously in both cells as the plasmid is being transferred, as one strand of the dna is being transferred, the other strand acts as a template for dna replication. Episome: genetic material not essential for host (such as plasmids). Is elements are transposons and the sites allow episomes to get integrated into the chromosome. Is elements are 800-1400 bp long: they have to be in both episomes and the chromosome for recombination to happen between the. Is elements: at the ends of is elements are terminal inverted repeats, most transposons have terminal inverted repeats, they play a big role in the transposition.
