BIOL1002 Lecture Notes - Lecture 2: Aequorea Victoria, Soil Salinity, Genetic Variation

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Genes that provide clear evidence that transformation has taken place and should confer a feature that does not occur in the host plant eg. change in colour enabling visualisation like the green fluorescent protein. The most widely used reporter gene isolated from the jellyfish aequorea victoria. First utilised as a reporter gene in 1994. Promoter: directs where, when and how much of the gene is produced. Coding sequence: downstream of promoter and encodes the desired protein e. g. gfp. Soil salinity affects large areas of crop land and is a major problem causing significant reductions in yield. On salty soils plants take up too much na+ which leads to toxicity and cell death. Approach: genetically modify arabidopsis plants to excrete more. Overexpression of a plant na+ transporter gene in the roots of arabidopsis plants. Result: plants are able to maintain growth in salty solution.

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