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Class Notes for DOH133 at Charles Sturt University

Oral Microbiology

DOH133 Lecture Notes - Lecture 12: Chitin, Facultative Anaerobic Organism, Cell Wall
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Identify two beneficial and two harmful effects of fungi Harmful effects of Fungi 1. Mycoses: Ringworm, athletes foot: caused by ascomycetes Trichophyton 2. Allergies: Sick building syndrome Stachybotrys chartarum 3. Toxin...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 11: Arthropod, Asexual Reproduction, Protozoa
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Summary outcomes of lytic and lysogenic life cycles Lytic cycle The bacteriophage enters the host cell, produces new particles and lyses the bacterial cell, causing death of the bacterium. Lysogenic cycle Can form a proph...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 10: Reverse Transcription Polymerase Chain Reaction, Bacteriophage, Lytic Cycle
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Differentiate a virus from a bacterium Virus characteristics 1. Obligatory intracellular parasites 2. Contain DNA or RNA 3. Contain a protein coat 4. Some are enclosed by an envelope 5. Some viruses have spikes 6. Most vir...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 9: Cementum, Endodontics, Dentin
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PBL 1. How antibiotic works lev 5 used to treat a variety of bacterial infections. Belongs to fluoroquinolone antibiotics. Works by stopping growth of bacteria. Does not effect viralinfections. Used to treat bacteria infec...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 8: Fluorophore, Refraction, Green Fluorescent Protein
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List the three domains of life 1. Bacteria 2. Archaea 3. Eukarya Some key contributors to microbiology history Ancestors of archaea bacteria were the first life on Earth 1665 Robert Hooke coined the term Cells to descri...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 7: Paramecium, Peptidoglycan, Prokaryote
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Explain how microbiology integrates into your course of study Formation of plaque (biofilms) Prevention of dental caries Causes of periodontal disease Antibiotic spectrum and modeofaction Good hygiene in clinical prac...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 6: Asepsis, Commensalism, Coevolution
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Describe each of the phases of biofilm formation 1. Pellicle formation Oral bacteria attach to pellicle 2. Transport Microbes approach tooth via salivary flow 3. Longrange interactions Balance between Van der Waals forces ...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 5: Glycogen, Dental Caries, Flaccid Paralysis
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Describe five ways that saliva modulates bacterial growth Waste removal The physical flow of saliva helps remove diseasecausing bacteria from teeth amp; soft oral surfaces Barrier amp; Buffer agent saliva contains bufferin...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 4: Disinfectant, Asepsis, Dental Plaque
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Describe how the LAL assay works LAL assay for endotoxin detection Limulus Amoebocyte Lysate Assay (LAL) LAL works by using WBC from the horse shoe crab. These WBCs are used because they are different from human WBC in th...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 3: Prostaglandin, Escherichia Coli, Thermostat
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Explain the methods of disease transmission 1. Contact Transmission Direct requires close association between infected and susceptible host Indirect fomites living on items Droplets 2. Vehicle transmission of disease F...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 2: Bacteremia, Chronic Wasting Disease, Echinococcosis
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List the four properties that define a virus. What is a virion? A Virus 1. Contains DNA or RNA 2. Has protein coat surrounding the nucleic acid 3. Multiplies inside a living cell using the synthetic machinery ...

DOH
DOH133
Bryant
DOH133 Lecture Notes - Lecture 1: Prophage, Animal Virus, Chromosome
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List 5 differences between viruses and bacteria 1. Bacteria have ribosomes, viruses do not 2. Bacteria have a cell wall, viruses have a protein coat 3. Bacteria are larger (1000nm), Viruses (20400nm) 4. Bacteria are unicel...

DOH
DOH133
Bryant

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