SLE206 Lecture Notes - Lecture 1: Phase-Contrast Microscopy, Confocal Microscopy, Fluorescence Microscope

• School: Deakin University
• Department: Science, Engineering and Built Environment
• Course: SLE206
• Professor: Jessica Gibbons

Document Summary

To appreciate the universal features of cells: variety of shapes and sizes dependent on function, all cells divide by mitosis, all living cells obey the central dogma of gene expression (see below) To be familiar with the process of gene expression. Dna synthesis (replication) rna synthesis (transcription) protein synthesis (translation) amino acid. To understand the features and operation of a light microscope. Internal structures are visible but only slight detail. Can only see outline of nucleus until cells are fixed and stained. Light microscopy: uses visible light to detect small objects. Phase contrast microscopy: cells are easier to see due to differences in refractive indices and varying thickness which cause a phase shift of light passing through the cell. Fluorescent microscopy: uses staining to see greater detail of internal structures. Electron microscopy: uses a beam of electrons to create the image. Much higher magnification and greater resolving power enabling to see smaller objects in finer detail.