BCMB20005 Lecture 6: Lecture 6
2 Aug 2018
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Document Summary
Protein chemistry and molecular biology: gene or cdna, transcription of dna into rna a. Insert into expression vector: translate into protein, transform e. coli or other host cell, get encoded protein. Or can go the other way: protein, determine amino acid sequence, synthesize dna proves, screen dna library by southern blotting. Identify gene or cdna that encodes that protein. Gene isolation (what genes encode that protein?) Proteins are linear polymers of monomer units (amino acids) Linked end to end via peptide (amide) bond: Carboxyl group bonds with -amino group via a condensation reaction. Folding up of folding to 3d structure. Structure of enzymes is critical to their catalytic activity. Important to consider primary, secondary structure, tertiary, quaternary as they are critical to catalytic activity. Increase rate of reaction by a factor of 10 17. Some require co-factors, coenzymes some work alone. Covalent bonds are re-arranged during an enzyme catalysed reaction.
