BCMB30004 Lecture Notes - Lecture 13: Lone Pair, Phosphorylase, Ribose

• School: University of Melbourne
• Department: Biochemistry and Molecular Biology
• Course: BCMB30004
• Professor: Professor Cheng

Document Summary

Dephosphorylation & protein kinase strctures and functions 1: * human kinome: kinases expressed in the human genome. There are ~ 500 kinases in humans and. 16% are protein tyrosine kinases a(cid:374)d that"s (cid:449)hy i(cid:374)side the (cid:272)ell there is (cid:373)u(cid:272)h lo(cid:449)er phosphorylatio(cid:374) of tyrosine residues in cellular proteins, most of the phosphorylation is in serine & threonine residues. In addition to protein kinases, there are also other types of kinases e. g. phospholipid kinase. Classification and families of protein kinases: human kinome: * the various protein kinases can be classified in the human genome by their functions and also by their sequence similarities. * there are 7 families in the human kinome: tk/tyrosine kinase, agc/pka pkg pkc, Camk/calmodulin dependent protein kinase e. g. phosphorylase kinase, cmgc, tkl, ste, cki. Structure and regulation of protein kinases pka as an example: * pka is tetramer and when it is in the tetrameric (2 regulatory subunits & 2 catalytic subunits) form it is inactive.