BCMB20005 Lecture 5: Summary Notes
Document Summary
All figures from lecture 5 slides, a willems-jones, march 2017, melbourne university (unless stated) How enzymes lower the activation energy to catalyse a reaction. The meaning of the term enzyme kinetics" to biochemists. The following terms: km (michaelis constant), vo (initial velocity of an enzyme-catalysed reaction, vmax (maximum velocity) How to determine km, vo and vmax experimentally using two graphical methods to handle experimental data. Links dna with protein via transcription of rna and translation into the protein via ribosomes. Protein chemistry and molecular biology are linked and rely on each other. Gene isolation: taking a protein and working backwards. Structure determination: crystalize the protein, and identify how it interacts with coenzymes and cofactors, such as how the structure changes, etc. Determination of mechanism of action: characterization of catalytic mechanisms, protein-ligand interactions. Industrial and/or medical applications, e. g. relenza: inhibitor of neuraminidase, protein biochem techniques was used to find how relenza can be used to treat influenza.