Fate Mapping Using a Fluorescent Dye:
Fate mapping is a method of understanding the embryonic origin of various
tissues in the adult organism by establishing the correspondence between
individual cells (or groups of cells) at one stage of development, and their
progeny at later stages of development.
Components of the Cytoskeleton:
- Microfilaments (actin filaments)
- Intermediate filaments
These are the thinnest filaments of the cytoskeleton. They are composed of linear
polymers of actin subunits, and generate force by elongation at one end of the
filament coupled with shrinkage at the other, causing net movement of the
intervening strand. They also act as tracks for the movement of myosin
molecules that attach to the microfilament and ‘walk’ along them.
These filaments are more stable (strongly bound) than actin filaments. Like actin
filaments, they function in the maintenance of cell shape by bearing tension
(microtubules, by contrast, resist compression. Think of micro-and intermediate
filaments as cables, and of microtubules as cellular support beams). Intermediate
filaments organize the internal tridimensional structure of the cell, anchoring
organelles and serving as structural components of the nuclear lamina and
sarcomeres. They also participate in some cell-cell and cell-matrix junctions.
Microtubules are hollow cylinders, most commonly comprising of 13
protofilaments which, in turn, are polymers of alpha and beta tubulin.