BIO282 Lecture Notes - Lecture 28: Dna-Binding Domain, Antennapedia, Repressor

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22 Jun 2018
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Learning objectives:
In this topic/lecture you will learn about:
Strategies to understand the molecular mechanisms of biological processes.
Embryo development in Drosophila
Role of maternal, segmentation and homeotic genes in development
The hox genes in Drosophila
How hox genes function and how they are controlled.
How we start from a single cell and become a multi-cellular organism
Cells of different tissues vary in their structure, composition and function.
All cells have the same genetic material then how does it happen?
It is possible by a differential gene expression;
(cells in different tissues express different set of genes)
How does a single cell (fertilized egg) differentiate into a complex multicellular
organism?
Drosophila is used as a system to study development because
It has a very short life cycle.
An egg develops into a complete adult in a period of only 9 days
Pattern Formation in Drosophila Serves as a Model for the Genetic Control of Development
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Everything is decided in regards to future development for what part of the egg produces
what part of the adult body
If differentiation is controlled by genes, it must be possible to isolate mutants whose
embryos are defective in differentiation.
Cell; normal differentiating capability
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Mutant; no differentiating capability
Proteins required for the embryo development of Drosophila
Identify the cytoplasm that complements the defect.
Fractionate proteins.
Identify protein(s) that are required at a particular stage of development.
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Document Summary

Drosophila is used as a system to study development because. It has a very short life cycle: an egg develops into a complete adult in a period of only 9 days. Pattern formation in drosophila serves as a model for the genetic control of development: everything is decided in regards to future development for what part of the egg produces what part of the adult body. If differentiation is controlled by genes, it must be possible to isolate mutants whose embryos are defective in differentiation. Proteins required for the embryo development of drosophila. Identify the cytoplasm that complements the defect: fractionate proteins. Mutations affecting embryo development are defined by three groups of genes: maternal genes/egg polarity genes, body itself, segmentation genes, head, thorax, lower body, homeotic genes, antenae, wings, etc. Fate of a cell is largely determined by its position in the egg. Cells at the anterior end develop into head structures.

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