LQB186 Lecture Notes - Lecture 5: Endergonic Reaction, Conformational Change, Exergonic Process

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peachguinea-pig552

9 Aug 2018

School

QUT

Department

Biomedical Sciences

Course

LQB186

Professor

Sally-Anne Stephenson

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Document Summary

An enzyme is usually a protein molecule with a characteristic sequence of amino acids that fold to produce a specific three-dimensional structure, which gives the molecule unique properties. Enzymes are globular proteins what work as catalysts they speed up chemical reactions without being altered themselves. Enzymes are often called biological catalysts, they are made by living cells and speed up biochemical reactions. The substances that enzymes convert into products in these reactions are called substrates. A general equation for an enzyme is: substrate enzyme product. Enzymes are found in all living cells and are also secreted by some cells to work outside. Many different enzymes are needed as reactions take place in cells nearly all of which need to be catalysed. Enzyme-substrate specificity is a significant difference between enzymes and non-biological catalysts such as the metals that are used in catalytic convertors of vehicles.

Related Questions

What are the correct answers to the parts of this quiz marked incorrect? (that is my one question. Please take your time. I value accuracy over speed. )

IncorrectQuestion 2

0 / 1 pts
Studies done by Buchner showed that ground-up yeast cells were able to convert sugar to alcohol. The components of the mixture that were responsible for this transformation were

DNA molecules.

enzymes.

lipids.

carbohydrates.

Bloom's Level: 2. Understand
Learning Outcome: 06.01
Section: 06.01
Topic: History of Microbiology

IncorrectQuestion 3
0 / 1 pts
Bacteria that can absorb light energy and convert it into ATP are commonly called

cytochromes.

lysosomes.

chemotrophs.

phototrophs.

cytochromes AND chemotrophs.

Bloom's Level: 1. Remember
Learning Outcome: 06.02
Section: 06.01
Topic: Microbial Metabolism

IncorrectQuestion 4
0 / 1 pts
The readily usable energy currency of cells is

electricity.

the electron transport system.

ATP.

CTP.

the electron transport system AND CTP.

Bloom's Level: 1. Remember
Learning Outcome: 06.02
Section: 06.01
Topic: Microbial Metabolism

IncorrectQuestion 5
0 / 1 pts
NAD in its reduced form is symbolized as

NADH.

NAD.

NAD-.

NADP.

Bloom's Level: 1. Remember
Learning Outcome: 06.03
Section: 06.01
Topic: Microbial Metabolism

IncorrectQuestion 6
0 / 1 pts
The most common starting pathway for the breakdown of sugars is

respiration.

fermentation.

glycolysis.

oxidation.

Bloom's Level: 2. Understand
Learning Outcome: 06.04
Section: 06.01
Topic: Microbial Metabolism

IncorrectQuestion 7
0 / 1 pts
Which of the following processes generates the greatest amount of energy?

fermentation

aerobic respiration

the Entner-Duodoroff pathway

glycolysis

fermentation AND the Entner-Duodoroff pathway

Bloom's Level: 2. Understand
Learning Outcome: 06.05
Section: 06.01
Topic: Microbial Metabolism

IncorrectQuestion 8
0 / 1 pts
Fermentation

uses an inorganic molecule as the final electron acceptor.

uses an organic molecule as the final electron acceptor.

results in the production of a large amount of ATP.

is necessary in some organisms to produce reduced electron carriers.

uses an organic molecule as the final electron acceptor AND is necessary in some organisms to produce reduced electron carriers.

Bloom's Level: 2. Understand
Learning Outcome: 06.05
Section: 06.01
Topic: Microbial Metabolism

IncorrectQuestion 9
0 / 1 pts
The use of the suffix "ase" on a word denotes a(n)

substrate.

product.

enzyme.

electron acceptor.

substrate AND electron acceptor.

Bloom's Level: 1. Remember
Learning Outcome: 06.06
Section: 06.02
Topic: Microbial Metabolism

IncorrectQuestion 10
0 / 1 pts
Enzymes speed up reactions by

raising activation energy.

producing heat.

reducing entropy.

lowering activation energy.

raising activation energy AND reducing entropy.

Bloom's Level: 2. Understand
Learning Outcome: 06.06
Section: 06.02
Topic: Microbial Metabolism

IncorrectQuestion 11
0 / 1 pts
Most enzymes

are generalists and typically recognize a number of different substrates.

are specialists and typically recognize a single substrate.

are active over a wide pH range.

have several active sites.

are generalists and typically recognize a number of different substrates AND have several active sites.

Bloom's Level: 2. Understand
Learning Outcome: 06.06
Section: 06.02
Topic: Microbial Metabolism

IncorrectQuestion 12
0 / 1 pts
Allosteric enzymes

may bind two substrates.

are used to bind to other enzymes.

have an additional binding site that is involved in regulating enzyme activity.

are twice as fast as single site enzymes.

may bind two substrates AND are twice as fast as single site enzymes.

Bloom's Level: 2. Understand
Learning Outcome: 06.09
Section: 06.02
Topic: Microbial Metabolism

Question 13
1 / 1 pts
Feedback inhibition

is a means of regulating the amount of product produced.

often involves the use of allosteric enzymes.

involves inhibiting the last of a series of reactions.

results in raising the activation energy for the reaction.

is a means of regulating the amount of product produced AND often involves the use of allosteric enzymes.

Bloom's Level: 2. Understand
Learning Outcome: 06.10
Section: 06.02
Topic: Microbial Metabolism

IncorrectQuestion 14
0 / 1 pts
Coenzymes are derivatives of

minerals.

proteins.

lipids.

vitamins.

Bloom's Level: 1. Remember
Learning Outcome: 06.07
Section: 06.02
Topic: Microbial Metabolism

Question 15
1 / 1 pts
Environmental factors that may affect enzyme activity include

temperature

pH

salt

age

temperature, pH AND salt

Bloom's Level: 2. Understand
Learning Outcome: 06.08
Section: 06.02
Topic: Microbial Metabolism

Question 16
1 / 1 pts
Most enzymes function best at

acidic pH and high salt concentrations.

basic pH and low salt concentrations.

neutral pH and high salt concentrations.

slightly above pH 7 and low salt concentrations.

Bloom's Level: 2. Understand
Learning Outcome: 06.08
Section: 06.02
Topic: Microbial Metabolism

Question 17
1 / 1 pts
Mercury in mercurochrome inhibits growth

through competitive inhibition.

through a reversible action.

by oxidizing the sulfhydryl groups in cysteine.

by changing the shape of proteins.

by oxidizing the sulfhydryl groups in cysteine AND by changing the shape of proteins.

Bloom's Level: 3. Apply
Learning Outcome: 06.10
Section: 06.02
Topic: Microbial Metabolism

IncorrectQuestion 18
0 / 1 pts
The term precursor metabolite refers to molecules that

activate cell components.

are used in biosynthesis.

result from cell activities.

are present but inactive.

Bloom's Level: 2. Understand
Learning Outcome: 06.11
Section: 06.03
Topic: Microbial Metabolism

IncorrectQuestion 19
0 / 1 pts
Glycolysis

requires oxygen.

produces 3 ATP molecules.

produces 4 molecules of NAD.

may occur under aerobic or anaerobic conditions.

produces 3 ATP molecules AND produces 4 molecules of NAD.

Bloom's Level: 2. Understand
Learning Outcome: 06.12
Section: 06.03
Topic: Microbial Metabolism

IncorrectQuestion 20
0 / 1 pts
The transition step

links glycolysis to the pentose phosphate pathway.

links the pentose phosphate pathway to the Entner-Duodoroff pathway.

links glycolysis to the TCA cycle.

takes place in the matrix of the nucleus.

Bloom's Level: 1. Remember
Learning Outcome: 06.11
Section: 06.03
Topic: Microbial Metabolism

Question 21
1 / 1 pts
The TCA cycle produces

ATP through substrate-level phosphorylation.

NADH.

FADH2.

precursor metabolites.

All of the choices are correct.

Bloom's Level: 1. Remember
Learning Outcome: 06.12
Section: 06.03
Topic: Microbial Metabolism

Question 22
1 / 1 pts
The electron transport system

requires a membrane.

utilizes the nuclear membrane of eukaryotes.

utilizes the mitochondrial membrane of prokaryotes.

generates a concentration gradient of protons.

requires a membrane AND generates a concentration gradient of protons.

Bloom's Level: 2. Understand
Learning Outcome: 06.13
Section: 06.04
Topic: Microbial Metabolism

IncorrectQuestion 23
0 / 1 pts
Which type of phosphorylation does not require a membrane?

Substrate-level phosphorylation

Oxidative phosphorylation

Photophosphorylation

All types of phosphorylation require a membrane.

Bloom's Level: 2. Understand
Learning Outcome: 06.13
Section: 06.04
Topic: Microbial Metabolism

Question 24
1 / 1 pts
Which pairing is incorrect?

A:T

G:C

A:U

A:G

Bloom's Level: 3. Apply
Learning Outcome: 07.01
Section: 07.01
Topic: Genetics

Question 25
1 / 1 pts
RNA is characterized by which one of the following features?

Deoxyribose.

Thymine.

Ribose.

Double-stranded.

Bloom's Level: 1. Remember
Learning Outcome: 07.01
Section: 07.01
Topic: Genetics

Question 26
1 / 1 pts
DNA is characterized by which of the following feature(s)?

Ribose.

Single-stranded.

Deoxyribose.

Thymine.

Deoxyribose AND thymine.

Bloom's Level: 1. Remember
Learning Outcome: 07.01
Section: 07.01
Topic: Genetics

Question 27
1 / 1 pts
Which of the following is/are true of DNA replication?

It starts at the origin of replication.

Nucleotides are added to the 3' end.

It requires an RNA primer to get started.

It utilizes polymerases.

All of the choices are true.

Bloom's Level: 2. Understand
Learning Outcome: 07.03
Section: 07.02
Topic: Genetics

Question 28
1 / 1 pts
Which is true about the RNA transcript?

It is formed using the DNA minus strand as a template.

It has the same 5'-3' orientation as the DNA positive strand.

It is made in short fragments that are then stitched together.

The template starts at the promoter region.

It is formed using the DNA minus strand as a template, it has the same 5'-3' orientation as the DNA positive strand AND the template starts at the promoter region.

Bloom's Level: 2. Understand
Learning Outcome: 07.04
Section: 07.03
Topic: Genetics

IncorrectQuestion 29
0 / 1 pts
How many nucleotides are in a codon?

1

2

3

4

5

Bloom's Level: 1. Remember
Learning Outcome: 07.05
Section: 07.03
Topic: Genetics

Question 30
1 / 1 pts
Which may be or is an RNA molecule?

AGCCTAC

GGGCCCA

GCCCUUA

AGCCTAC AND GGGCCCA

GGGCCCA AND GCCCUUA

Bloom's Level: 2. Understand
Learning Outcome: 07.01
Section: 07.01
Topic: Genetics

IncorrectQuestion 31
0 / 1 pts
Which molecule carries an anticodon?

DNA

mRNA

rRNA

tRNA

Bloom's Level: 1. Remember
Learning Outcome: 07.05
Section: 07.03
Topic: Genetics

Question 32
1 / 1 pts
AUG

is only used as the start codon.

codes for methionine.

determines the reading frame.

is one of the stop codons.

codes for methionine AND determines the reading frame.

Bloom's Level: 2. Understand
Learning Outcome: 07.05
Section: 07.03
Topic: Genetics

IncorrectQuestion 33
0 / 1 pts
The A-site

is found on the RNA polymerase enzyme.

is found on the 30S ribosome.

is found on the 70S ribosome.

is the amino acid site.

is found on the 70S ribosome AND is the amino acid site.

Bloom's Level: 1. Remember
Learning Outcome: 07.05
Section: 07.03
Topic: Genetics

IncorrectQuestion 34
0 / 1 pts
A stop codon

codes for the stop amino acid s-methyl-methionine.

forms a hairpin loop forcing the ribosome to fall off.

codes for no amino acid.

enhances the binding of the e-polymerase.

codes for the stop amino acid s-methyl-methionine AND forms a hairpin loop forcing the ribosome to fall off.

Bloom's Level: 2. Understand
Learning Outcome: 07.05
Section: 07.03
Topic: Genetics

IncorrectQuestion 35
0 / 1 pts
Some segments of the precursor mRNA in eukaryotes are non-coding and are called

exons.

introns.

integrans.

uselessans.

Bloom's Level: 1. Remember
Learning Outcome: 07.06
Section: 07.04
Topic: Genetics

Question 36
1 / 1 pts
Gene regulation may entail

turning on genes only when needed.

turning off genes when not needed.

turning on or off entire groups of genes.

All of the choices are correct.

Bloom's Level: 2. Understand
Learning Outcome: 07.09
Section: 07.06
Topic: Genetics

IncorrectQuestion 37
0 / 1 pts
The set of genes in bacteria that are linked together and transcribed as a single unit is referred to as a(n)

operon.

regulon.

operator.

repressor.

Bloom's Level: 1. Remember
Learning Outcome: 07.09
Section: 07.06
Topic: Genetics

Question 38
1 / 1 pts
The molecules that bind to a repressor and cause it to no longer bind to the operator are called

activators.

repressors.

introns.

inducers.

Bloom's Level: 2. Understand
Learning Outcome: 07.09
Section: 07.06
Topic: Genetics

Question 39
1 / 1 pts
Repressors

are involved in negative control.

are involved in positive control.

always bind to the promoter.

bind or do not bind to the operator depending on their shape (conformation).

are involved in negative control AND bind or do not bind to the operator depending on their shape (conformation).

Bloom's Level: 2. Understand
Learning Outcome: 07.09
Section: 07.06
Topic: Genetics

Question 40
1 / 1 pts
Activators

are involved in negative control.

are involved in positive control.

always bind to the promoter.

are allosteric proteins.

are involved in positive control AND are allosteric proteins.

Bloom's Level: 2. Understand
Learning Outcome: 07.09
Section: 07.06
Topic: Genetics

Question 41
1 / 1 pts
Signal transduction

is the relay of information about conditions outside a cell to inside the cell.

often relies on a two component system.

may involve phosphorylation of various molecules.

is used by certain pathogens to sense low magnesium conditions.

All of the choices are correct.

Bloom's Level: 2. Understand
Learning Outcome: 07.07
Section: 07.05
Topic: Genetics

IncorrectQuestion 42
0 / 1 pts

Quorum sensing

is used by bacteria to sense the density of cells.

is used by bacteria to sense the proximity of eukaryotic cells

is used by bacteria to limit the density of cells.

is used by eukaryotes to sense the presence of bacteria.

does not influence the expression of genes by a bacteria.

Quorum sensingBloom's Level: 2. UnderstandLearning Outcome: 07.07Section: 07.05Topic: Genetics

Question 43
1 / 1 pts
In DNA sequence analysis

the + strand of DNA is used.

the start codon is ATG.

ORFs are searched for.

codon usage is a helpful indicator for protein coding areas.

All of the choices are correct.

Bloom's Level: 2. Understand
Learning Outcome: 07.12
Section: 07.08
Topic: Genetics

IncorrectQuestion 44
0 / 1 pts
DNA replication is usually unidirectional.

True

False

Bloom's Level: 2. Understand
Learning Outcome: 07.03
Section: 07.02
Topic: Genetics

IncorrectQuestion 45
0 / 1 pts
There are 4 functional types of RNA.

True

False

Bloom's Level: 1. Remember
Learning Outcome: 07.04
Section: 07.03
Topic: Genetics

Question 46
1 / 1 pts
Antisense RNA is the complement of the plus strand and may be useful in inhibiting translation.

True

False

Bloom's Level: 2. Understand
Learning Outcome: 07.11
Section: 07.07
Topic: Genetics

IncorrectQuestion 47
0 / 1 pts
A drug that inhibits the activity of bacterial DNA gyrase ______ be a good antibiotic because _______.

would; it would inhibit all DNA synthesis

would NOT; it would inhibit all DNA synthesis, even our own cell's DNA synthesis

would; it would selectively inhibit bacterial DNA synthesis due to differences between bacterial/human gyrase

would NOT; the DNA replication machinery could still function, even with impaired DNA gyrase enzyme

Bloom's Level: 5. Evaluate
Learning Outcome: 07.03
Section: 07.02
Topic: Genetics

IncorrectQuestion 48
0 / 1 pts
The regulatory protein

binds to the promoter region of DNA.

may inhibit or enhance transcription.

may control translation of the operon.

affects the activity of the DNA polymerase.

binds to the promoter region of DNA AND affects the activity of the DNA polymerase.

Bloom's Level: 2. Understand
Learning Outcome: 07.09
Section: 07.06
Topic: Genetics

Question 49
1 / 1 pts
The source of variation among microorganisms that were once identical is

antibiotic resistance.

virulence factors.

sigma factors.

mutation.

Bloom's Level: 1. Remember
Learning Outcome: 08.02
Section: 08.01
Topic: Genetics

Question 50
1 / 1 pts
The characteristics displayed by an organism in any given environment is its

genotype.

archaetype.

mutatotype.

phenotype.

Bloom's Level: 2. Understand
Learning Outcome: 08.01
Section: 08.01
Topic: Genetics

Question 51
1 / 1 pts
Transposons

are informally known as jumping genes.

may cause insertion mutations.

may cause knockout mutations.

were first recognized in plants.

All of the choices are correct.

Bloom's Level: 2. Understand
Learning Outcome: 08.05
Section: 08.02
Topic: Genetics

Question 52
1 / 1 pts
The largest group of chemical mutagens consists of

radiation.

base analogs.

nitrous acid.

alkylating agents.

Bloom's Level: 1. Remember
Learning Outcome: 08.06
Section: 08.03
Topic: Genetics

Question 53
1 / 1 pts
Nitrous acid most frequently causes mutations by

substituting oxygen for hydrogen bonds.

converting keto groups to amino groups.

altering the bonding ratios of nitrogen bases.

converting cytosine to uracil.

Bloom's Level: 2. Understand
Learning Outcome: 08.06
Section: 08.03
Topic: Genetics

IncorrectQuestion 54
0 / 1 pts
Chemical mutagens that mimic the naturally occurring bases are called

nitrogen mustards.

alkylating agents.

base analogs.

nitrous oxide.

Bloom's Level: 1. Remember
Learning Outcome: 08.06
Section: 08.03
Topic: Genetics

IncorrectQuestion 55
0 / 1 pts
Irradiation of cells with ultraviolet light may cause

4 nucleotides to covalently bind together.

thymine dimers.

adenine complementary base pairing with cytosine.

the addition of uracil.

Bloom's Level: 2. Understand
Learning Outcome: 08.08
Section: 08.03
Topic: Genetics

Question 56
1 / 1 pts
Thymine dimers are dealt with by

no repair mechanisms.

photoreactivation repair.

SOS repair.

excision repair.

photoreactivation repair AND excision repair.

Bloom's Level: 2. Understand
Learning Outcome: 08.11
Section: 08.04
Topic: Genetics

IncorrectQuestion 57
0 / 1 pts
X-rays

have no effect on DNA.

cause thymine trimers.

cause single and double strand breaks in DNA molecules.

make the DNA radioactive.

Bloom's Level: 1. Remember
Learning Outcome: 08.08
Section: 08.03
Topic: Genetics

IncorrectQuestion 58
0 / 1 pts
DNA repair mechanisms occur

only in prokaryotes.

only in eukaryotes.

in both eukaryotes and prokaryotes.

in neither eukaryotes or prokaryotes.

Bloom's Level: 1. Remember
Learning Outcome: 08.09
Section: 08.04
Topic: Genetics

IncorrectQuestion 59
0 / 1 pts
Antibiotics

cause mutations to occur.

may act as alkylating mutagens.

provide an environment in which pre-existing mutants survive.

increase the rate of spontaneous mutation.

Bloom's Level: 2. Understand
Learning Outcome: 08.12
Section: 08.05
Topic: Genetics

IncorrectQuestion 60
0 / 1 pts
Prokaryotic cell mutations can be observed very quickly because the prokaryotic chromosome is

diploid.

polyploid.

haploid.

polysomal.

Bloom's Level: 2. Understand
Learning Outcome: 08.02
Section: 08.01
Topic: Genetics

Question 61
1 / 1 pts
The diploid character of eukaryotic cells may mask the appearance of a mutation since

this may be a frame shift.

the mutation is often reversible.

the mutation may be palindromic.

the matching chromosome may carry the dominant gene.

Bloom's Level: 2. Understand
Learning Outcome: 08.02
Section: 08.01
Topic: Genetics

IncorrectQuestion 62
0 / 1 pts
A clever technique that streamlines the identification of auxotrophic mutants is

gas chromatography.

replica plating.

direct selection.

reversion.

Bloom's Level: 2. Understand
Learning Outcome: 08.12
Section: 08.05
Topic: Genetics

IncorrectQuestion 63
0 / 1 pts
To increase the proportion of mutants in a population of bacteria one may use

direct selection.

replica plating.

penicillin enrichment.

individual transfer.

Bloom's Level: 2. Understand
Learning Outcome: 08.12
Section: 08.05
Topic: Genetics

Question 64
1 / 1 pts
Bacteria that have properties of both the donor and recipient cells are the result of

UV light.

SOS repair.

frame shift mutations.

genetic recombination.

Bloom's Level: 2. Understand
Learning Outcome: 08.13
Section: 08.06
Topic: Genetics

IncorrectQuestion 65
0 / 1 pts
The mechanism by which genes are transferred into bacteria via viruses is called

ellipsis.

replica plating.

transformation.

transduction.

conjugation.

Bloom's Level: 1. Remember
Learning Outcome: 08.14
Section: 08.07
Topic: Genetics

IncorrectQuestion 66
0 / 1 pts
In conjugation the donor cell is recognized by the presence of

an F plasmid.

a Y chromosome.

diploid chromosomes.

an SOS response.

an F plasmid AND diploid chromosomes.

Bloom's Level: 2. Understand
Learning Outcome: 08.15
Section: 08.08
Topic: Genetics

IncorrectQuestion 67
0 / 1 pts
Gene transfer that requires cell-to-cell contact is

transformation.

competency.

conjugation.

functional genomics.

Bloom's Level: 2. Understand
Learning Outcome: 08.15
Section: 08.08
Topic: Genetics

Question 68
1 / 1 pts
The transfer of vancomycin resistance from Enterococcus faecalis to Staphylococcus aureus is thought to have involved

conjugation.

transformation.

transduction.

transposons.

conjugation AND transposons.

Bloom's Level: 2. Understand
Learning Outcome: 08.17
Section: 08.09
Topic: Genetics

IncorrectQuestion 69
0 / 1 pts
Double-stranded DNA enters the recipient cell during transformation.

True

False

Bloom's Level: 2. Understand
Learning Outcome: 08.13
Section: 08.06
Topic: Genetics

Question 70
1 / 1 pts
Plasmids often carry the information for antibiotic resistance.

True

False

Bloom's Level: 2. Understand
Learning Outcome: 08.16
Section: 08.09
Topic: Genetics

Quiz Score: 31 out of 70

1. C6H12O6 + 6O2 Ã 6CO2 + 6H2O Glucose is being _____ to _____ and oxygen is being _____ to _____. a. oxidized; carbon dioxide; reduced; water b. oxidized; water; reduced; carbon dioxide Incorrect c. reduced; water; oxidized; carbon dioxide d. reduced; carbon dioxide; oxidized; water

2. Which of the following stages in the breakdown of the piece of toast you had for breakfast generates the most ATP? a. the citric acid cycle Incorrect b. glycolysis c. the digestion of starch to glucose d. oxidative phosphorylation

3. Which of the following is a catabolic pathway? a. gluconeogenesis b. production of starch Incorrect c. glycolysis d. production of glycogen

4. Per molecule, the oxidation of a fatty acid results in _____ energy storage than the oxidation of glucose. a. the same b. less Incorrect c. more d. None of the above; fatty acids cannot be oxidized.

5. Of the eight reactions in the citric acid cycle, how many are oxidation-reduction reactions? a. one b. two c. three d. four e. five

6. The process of glycolysis transfers the energy in sugar molecules into the stored energy of activated carriers such as ATP and NADH. Which statement is true about glycolysis? a. Only eukaryotic organisms do glycolysis. b. The ten reactions of glycolysis are identical in all eukaryotic cells, but are different in prokaryotic cells due to their diverse metabolic strategies. Incorrect c. The ten reactions of glycolysis are identical in almost all living organisms on the planet. d. There are many ways to oxidize glucose and transfer energy into activated carriers; the diversity of organisms on the planet is reflected in the diversity of their mechanisms of glycolysis.

7. Referring to the sentence completed in #13 above, the purpose of the latter reaction (in active muscle cells) is to _____. a. accumulate lactic acid in the cell, which provides additional energy for the production of ATP Incorrect b. slow down glycolysis until oxygen becomes available again c. accelerate the Krebs cycle, which will restore the oxygen supply d. regenerate NAD+ so that glycolysis can continue in the absence of oxygen

8. What are the products of the citric acid cycle? a. carbon dioxide, NADH, FADH2 and GTP b. carbon dioxide, NAD+, FAD and GTP c. NADH, FADH2, and acetyl CoA Incorrect d. carbon dioxide and water

9. Which statement is true about enzymes? a. Enzymes catalyze chemical reactions because they make inherently endergonic reactions exergonic. b. Each enzyme in a cell can catalyze many different chemical reactions involving chemically different substrates. Incorrect c. Enzymes can only catalyze reactions in one direction. d. After catalyzing a reaction, an enzyme is permanently altered and can no longer bind substrate. e. None of the above is true. 1

10. âG measures the change in free energy in a system as it converts reactant (Y) into product (X). When [X] = [Y], âG is equal to _____. a. RT b. ÎGÂ° + RT c. ÎGÂ° d. ln [X]/[Y]

11. The âGÂ° for the hydrolysis of ATP is -7.3 kcal/mol. What conditions in the cell would lead to a âG for ATP hydrolysis of -11 kcal/mol? a. An increase in the ATP/ADP ratio b. An increase in anabolic activity in the cell c. Transport of ATP out of the compartment where the reaction is occurring Incorrect d. A decrease in the ATP/ADP ratio

12. In the first reaction of glycolysis, a phosphate transferred from ATP to a glucose molecule to produce glucose-6-phosphate and ADP. Under conditions existing in the cell, the free energy change for this reaction is -8.0 kcal/mole. Which of the following statements is true? Recall that the âGÂ° for the hydrolysis of ATP is -7.3 kcal/mol. a. Glucose concentrations in the cell are always low, making this reaction more favorable than would be expected. b. This reaction is less favorable than would be expected, given the âGÂ° of ATP hydrolysis. c. Glucose-6-P concentrations in the cell are very low relative to glucose concentrations, making the reaction more favorable than would be expected. d. The âGÂ° for the addition of a phosphate to glucose must be -0.7 kcal/mole.

13. Protein E can bind to two different proteins, S and I. The binding reactions are described by the following equations and values: E + S --> ES Keq for ES = 10 E + I> EI Keq for EI = 2 Given the equilibrium constant values, which one of the following statements is true? a. When S is present in excess, no I molecules will bind to E. b. E binds I more tightly than S. c. The binding energy of the ES interaction is greater than that of the EI interaction. d. Changing an amino acid on the binding surface of I from a basic amino acid to an acidic one will probably make the free energy of association with E more negative.

1.When something appears blue, it is absorbing all colors except__________.

yellow

blue

red

green

orange

2.During heavy exercise, lactic acid can build up in ourmuscles. Why? How does this resemble the metabolism of bacteria andyeast?

lactic acid is results from insufficient oxygen during sugarmetabolism; it is fermentation

lactic acid accumulates when we are dehydrated; bacteria andyeast use the same pathway

lactic acid is built up from carbon dioxide; bacteria and yeaststore energy in this way

3.Why must a cell keep a similar concentration of dissolvedsubstances with the fluid surrounding them?

to avoid dilution of potential energy

to avoid osmotic imbalance and rapid gain or loss of water

to maintain the correct amount of dehydration and hydrolysis

4.What is entropy?

the amount of energy in a system

the amount of disorganization

the amount of organization

5.Burning a log is the reverse reaction found in photosynthesis.What products would be formed in burning a log?

carbon dioxide and water

glucose and oxygen

oxygen and carbon dioxide

oxygen and water

6.The main reason that cellular respiration needs to occur stepby step instead of a single, big reaction is

cells don't store enough oxygen

too much energy would be released for the cell to harness

cells don't have many mitochondria.

cells produce the enzymes needed for cellular respiration veryslowly

7.Enzymes are catalysts because they operate to:

raise activation energy

lower activation energy

supply activation energy

supply the reactants

8.When ATP powers a reaction it becomes ADP. The ADP

can reform ATP by an exergonic reaction

is a waste product for lysosomal breakdown

can reform ATP by an endergonic reaction

can reform ATP by an oxidation reaction

can reform ATP by an equilibrium reaction

9.The energy source that drives photosynthesis is

water

glucose

carbon dioxide

oxygen

sunlight

10. What is the process by which enzymes regulate their ownactivity?

osmosis

self-propulsion

feedback inhibition

11.Coupled reactions are reactions in which a

endergonic reaction drives a spontaneous reaction

exergonic reaction drives a spontaneous reaction

endergonic reaction drives an exergonic reaction

exergonic reaction drives an endergonic reaction

12.How is water used in photosynthesis?

as a substrate for ATP synthase

as an electron donor

to combine with ADP forming ATP

as an electron acceptor

13.Why are the âDark Reactionsâ of photosynthesis referred to asâDarkâ?

they do not require light

they can only occur at night

they generate excess sugar and the leaves turn brown

14.What is the difference between potential and kineticenergy?

potential is stored, kinetic is being used

potential is being used, kinetic is ready to be used

potential is negative, kinetic is positive

15.The products of cellular respiration are

carbon dioxide, water, and ATP

glucose, water, and ATP

glucose, carbon dioxide, and ATP

oxygen, ATP, and water

carbon dioxide, glucose, and water

16.All of the following are correct about energy except:

The biological world gets its energy from the sun.

Chemical bonds store potential energy.

A boulder perched on a hill has kinetic energy.

Energy is defined as the ability to do work.

All forms of energy can be converted to heat.

17.What is needed for the end product of glycolysis, pyruvate,to enter the citric acid cycle?

More glucose for energy

ATP

Oxygen

Carbon dioxide

18.The enzyme that forms a transmembrane channel in mitochondriaand phosphorylates ADP

a carrier protein

ATP synthase

acetyl CoA

19.Fermentation is most common in

plants

microorganisms

animals

20.During adsorption of sunlight by photosystems, H+ ions aregenerated. Where do they come from? What are they used for?

from the breakdown of sugar; they help form water

water; they help form sugar

from carbon dioxide; they help dissolve NaCl

21.Glycolysis

does not use oxygen

uses oxygen

uses carbon dioxide

22.Photosynthesis is

not dependent on chlorophyll

a process that produces glucose and oxygen

a process that produces water and carbon dioxide

23.Breakdown of glucose generates many ATP molecules, each ofwhich can be used independently. Why is ATP ideally suited to powerbiological reactions?

ATP has many hydrogen bonds

ATP has enough energy to power one biological reaction

ATP readily dissolves in water

24.Objects that are not actively moving but have the capacity todo so are said to possess:

kinetic energy

entropy

potential energy

living energy

25.The first stage of cellular respiration, called ___________,takes place in the cytoplasm of the cell and needs no oxygen.

photorespiration

citric acid cycle

glycolysis

oxidation

26.Diffusion

requires energy

utilizes proteins to move molecules across a membrane

moves molecules against a concentration gradient

does not require energy

cannot occur without a membrane present

27.What is the name of the molecule on which an enzymeworks?

substrate

monomer

polymer

28.ATP contains

phenylalanine

two phosphate groups

three nitrate groups

three phosphate groups

29.How does chlorophyll function in photosynthesis?

by absorbing carbon dioxide

by absorbing the sun's energy

by absorbing water

30.Objects that are moving are said to possess:

living energy

potential energy

entropy

kinetic energy

31.The respiration pathway that evolved first is probably

glycolysis

aerobic respiration

citric acid cycle

32.The energy source for the process of photosynthesis is

oxygen

sunlight

carbon dioxide

chlorophyll

glucose

33.Burning wood generates heat and light. What type of reactionis this?

exergonic

kinetic energy

endergonic

potential energy

equilibrium

34.What is meant by a âcoupled reactionâ in terms of energy?

many poymers are built simultaneously

energy released by one reactions powers another reaction

some reactions occur in the same organelle

35.The First Law of Thermodynamics states:

energy is constantly being created by entropy events

energy can be changed from one form to another, but cannot becreated or destroyed

energy can be used and then destroyed because of entropy'sactions on it

energy can be destroyed while it is producing entropy

36.How do we dispose of the carbon derived from the glucose thatis metabolized during respiration?

by breathing out

via our urine

it is broken down in lysosomes

37.Both plant and animal cells have mitochondria because theyboth

carry on respiration

carry on photosynthesis.

require water

38.What is Activation Energy?

the energy needed to initiate a reaction

the energy that transports water into cells

potential energy

39.If you put a plant in a chamber and shine a light on it

oxygen will decrease and carbon dioxide will increase

oxygen will increase and carbon dioxide will decrease

oxygen and carbon dioxide will increase

oxygen and carbon dioxide will decrease

40.What are the inputs and the output molecules for the overallphotosynthetic process?

input sugar and oxygen; output carbon dioxide and water

input oxygen and water; output sugar and carbon dioxide

input carbon dioxide and water;output sugar and oxygen

41.What kind of reaction is photosynthesis?

endergonic

kinetic energy

exergonic

potential energy

equilibrium

42.In the process of glycolysis, glucose is cleaved into a pairof three-carbon molecules called

lactate

pyruvate

acetyl CoA

ATP

alcohol

43.Entropy is

order

disorder

complexity

Both order and disorder are correct

Both complexity and disorder are correct

44.Glycolysis takes place in the _____________ and the citricacid cycle and electron transport chain take place in the___________.

cytoplasm; endoplasmic reticulum

mitochondria, chloroplast

mitochondria; cytoplasm

cytoplasm; mitochondria

45.The organelle that carries out photosynthesis in plants isthe

mitochondria

chloroplast

ribosome

chlorophylllysosome

46.The energy to power the Calvin cycle comes from

cellular respiration

oxygen

the light reactions of photosynthesis

47.Which of the following can be broken down into intermediateproducts that enter cellular respiration?

Proteins

Lipids

Carbohydrates

All of these.

48.When cellular product levels get too high, the responsibleenzyme is inhibited. This is an example of

promotion

denaturation

equilibrium

a coenzyme

feedback inhibition

49.How is respiration the opposite of photosynthesis?

respiration breaks sugar down to carbon dioxide; photosynthesisbuilds sugar from carbon dioxide

respiration builds sugar from carbon dioxide; photosynthesisbreaks sugar down to carbon dioxide

respiration requires energy input; photosynthesis gives offexcess energy

50.The molecule that absorbs sunlight for photosynthesis is

oxygen

carbon dioxide

chlorophyll

glucose

sunlight

amaranthbear841

LQB186 Lecture Notes - Lecture 5: Endergonic Reaction, Conformational Change, Exergonic Process

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