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BIOC2000 (32)

Class Notes for BIOC2000 at University of Queensland

Biochemistry & Molecular Biology

BIOC2000 Lecture Notes - Lecture 13: Isoelectric Point, Protein Structure, Peptide Bond

Protein Summary Tuesday, 7 June 2016 2:24 PM AA made up of Nterminus: on left (L AAs) in anabolic AAs charged amine (NH3+) at phys pH Cterminus: on right in anabolic AAs charged carboxylic acid (COO) at phys pH Rgroup: s...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 6: Stem-Loop, British Association For Immediate Care, Transfer Rna

PCR, DNA, RNA, Protein synthesis Sunday, 5 June 2016 9:35 PM PCR requires: template DNApol two primers dNTPs Steps: 1. denaturation (92C) 2. annealing 3. elongation (92C) primer design will be on exam, so: 1824 NTs lo...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 5: Helicase, Membrane Protein, Conformational Change

Entropy Monday, 6 June 2016 11:58 PM LAWS First law of thermodynamics energy remains constant within the universe or any reacting system. Energy cannot be created or destroyed. Second law of thermodynamics the natural te...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 3: Bond Length, Dissociation Constant, Electronegativity

Intramolecular forces Monday, 6 June 2016 11:23 PM DEFINITIONS van der Waals forces forces that exist between molecules of the same kind H bonding van der Waals force that gets a special name dipoledipole interaction oc...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 28: Oxyanion Hole, Conjugate Acid, Deprotonation
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Monday, 14 May, 2018 Gilam Lec How different AA contribute to catalysis (role) Acid base mechanism of electron pushing (DHAP Intermediate not very stable breaksdown to DA3P residue taking proton conjugate acid donating p...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 34: Acetyl-Coa, Cytochrome C Oxidase, Atp Synthase
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Monday, 28 May, 2018 Glucose 03 ETC Complex I II III IV V NADH Dehydrogenase transfer 2e to ubiquinnons (Cofactor embedded in ETC bilayer) U accept 2e and transport to Complex III Bc complex U either accept protons from FA...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 33: Acetyl-Coa, Citric Acid Cycle, Electrochemical Gradient
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Friday, 25 May, 2018 TCA cycle TCA Citric Acid Cycle Krebs Cycle located in mitochondria glucose fat protein centralised to pyruvate for TCA cycle entering Amphibolic (making and breaking role; synthesis and knocking ov...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 10: Hyperbola, Allosteric Regulation, Phosphofructokinase
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Friday, 18 May, 2018 Gilam L10 Allosteric regulation of enzyme glycolysis Phosphofructosekinase Hexokinase for glycogen synthesis Phosphohexose isomerase for gluconeogenesis shared pathway unique to glycolysis: PFK eff...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 6: Dna Replication, Activation Energy, Ribose
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Saturday, 9 June, 2018 Rowland ATP and Central Dogma delta g= delta H T x delta S High energy biomolecules take energy to make All biological system follows rst and second law of thermodynamics In second law of thermod...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 3: Lipid Bilayer, Beta Sheet, Micelle
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Saturday, 9 June, 2018 Susan L0304 Folding refers to protein backbone taking up space Weak forces drive folding (h bond and what Van der Waals force) Covalent bond restrict folding energy can be release when bond form o...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 26: Enzyme Catalysis, Cooperative Binding, Conformational Change

Lecture 26 Multiple weak, reversible interactions are important for enzyme catalysis 1. E binds S with some specificity to form encounter complex Binding (Step 1) process has been described in a few ways. st 1 model: Lo...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 25: Conjugate Acid, Acid Dissociation Constant, Amine

Lecture 25 *Be familiar with ways in which amino acids are ionized -COOH group (uncharged) ionizes to form COO (vely charged) therefore carboxyl group can exist in an uncharged state or vely charged state - Lysines pri...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture 24: Lecture 24

Lecture 24:Quantifying binding interactions Quantifying analysis of ES complex formation Rates of association and dissociation are determined by rate constants, kon (or k1) and offor k1 capital K equilibrium constants; low...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 23: Alpha Helix, Stereoselectivity, Nucleophile

Lecture 23:Enzymology Enzymes interact physically with their substrates - bind the substrate, transition state and product using weak interactions This binding causes: ENTHALPIC changes Favourable: Enthalpy decreases r...

Biochemistry
BIOC2000
Dr Justin Ridge
BIOC2000 Lecture Notes - Lecture 22: Boltzmann Distribution, Activation Energy, Kinetic Energy

Lecture 22:Enzymology Lecture 2 In a state function, the final state is independent of the path taken from the start e.g. in H, S, & G Activation energy: - The transition between two states requires a molecule to be con...

Biochemistry
BIOC2000
Dr Justin Ridge

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