BCMB20002 Lecture Notes - Lecture 13: Dna Polymerase Iii Holoenzyme, Dna Polymerase I, Dna Gyrase

• School: University of Melbourne
• Department: Biochemistry and Molecular Biology
• Course: BCMB20002
• Professor: Terry Mulhern

Document Summary

All figures from lecture 13 of h. verkade, melbourne university, march 2017 (unless stated) Explain what it means that dna replication is semi-conservative. Demonstrate why the replication of the two strands must be accomplished in very different ways. Describe the roles of the major proteins involved in dna replication. Explain the three-dimensional organisation of the replication fork. Explain the specific roles of dna pol i and dna pol iii in dna replication. Outline how the fidelity of dna replication is maintained, and why this is important. Chromosomes need to be replicated into two identical chromosomes before they cell replicates into two cells: coli has similar dna synthesis to humans. The hydrogen bonds between the base pairs tell how dna can be replicated, because only one strand is needed to replicate the dna. The second strand can be made due to complementary base pairing, and how many hydrogen bonds between the base pairs.