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Class Notes for BIOL3090 at University of Newcastle

Molecular Biology

BIOL3090 Lecture Notes - Lecture 19: Histone Deacetylase, Histone Code, H3K4Me3

Lecture 7: Chromatin Nucleosome made up of histones. Core is 8 histones H3, H4, H2A and H2B (two of each). H1 histones outside of nucleosome holds DNA to nucleosome not really sure how they sit. H3 and H4 dimerise to form ...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 21: Eif4E, Eukaryotic Initiation Factor 3, Eif2

Lecture 9: Transcription factories and translation Transcription factories several genes transcribed at RNAPII transcription factory. Contains capping, splicing and polyadenylation enzymesfactors in a cluster. Everything i...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 3: Adenylyl Cyclase, Lac Operon, Isomerization

Lecture 3 Lac if both glucose and lactose. Glucose = monosaccharide very simple sugar. Lactose = disaccharide Uses up simple sugars first. Then there is a lag change in transcriptional regulation. Diauxic growth. Bgalactos...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 9: World Federation Of Trade Unions, Molecular Mimicry, Inosine

Lecture 9: Translation Elongation and Termination Elongation factors EfTu and EfTs have an interdependent relationship. EfTu most abundant protein in a cell. EfTu in complex with GTP is able to bind an aatRNA it then deliv...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 17: Differential Centrifugation, Western Blot, Polyadenylation

Lecture 5: Capping and Polyadenylation Capping U1 being driven by two different promoters one that leads to capping and another that doesnt. They looked at where the RNA is located in Xenopus oocytes. In wt with cap it is ...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 10: Signal Transduction, Starvation Response, Quorum Sensing

Lecture 10: Signal transduction Signal transduction: Interpretation of an external signal via phosphorproteins to elicit a molecular response within the cell through modulating the phosphorylation site of a protein. Simple...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 13: Tata Box, Transcription Preinitiation Complex, Transcription Factor Ii A

Lecture 3: GTFs and TAFs GTFsimportant for initiation.Eukaryotesare weak at initiationtranscription so they use GTFs. Them together forms the preinitiation complex (PIC). DABPolF complex determined through a gel shift assa...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 1: Dna Ligase, Photographic Emulsion, Alpha Particle

Section 1 Prokaryotes Lecture 1: DNA replication Isotopes reveal Semiconservative MesselinStahl. Used two different isotopes 15N and 14N. Could physically separate based on weight due to 15N heaviness. separate through Ce...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 7: Single Particle Analysis, Prokaryotic Small Ribosomal Subunit, Aminoacyl-Trna

Lecture 7: Ribosome structure. 70s = 30s and 50s. Negative stain single particle analysis, end up with metal shell around protein ribosome. rRNA forms bulk of ribosome. rRNA forms binding sites for tRNA molecules. Active s...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 5: Antitermination, Lysogenic Cycle, Bacteriophage

Lecture 5: Molecular switches: Lysis Vs Lysogeny Lytic phase: Phage infection, phage replication, phage assembly, lysis. Lysogenic phase: occurs when an alternate transcription event happens (at the decision point). Phage ...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 15: Leucine Zipper, Dna-Binding Domain, Glucocorticoid Receptor

Lecture 4: Transcription Factors Not all genes need to be expressed in all settings. What drives that are activators. Can stimulate or inhibit genes. Are composed of at least 2 functional domains. Must have DNAbinding doma...

Biological Sciences
BIOL3090
Peter Lewis
BIOL3090 Lecture Notes - Lecture 11: Tata Box, Ribosomal Rna, Nucleoplasm

Part 2 Eukaryotes Lecture 1: Overview and Promoters Transcription 3 type of promoters 3 types of polymerase Focus on pol II promoters and polymerase enhancerssilencers Transcription factors General transcription facto...

Biological Sciences
BIOL3090
Peter Lewis

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