🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
HomeClass Notes1,200,000AU40,000

BIOL1003 Lecture Notes - Lecture 15: Phenotype, Allele, Mendelian Inheritance

41 views19 pages
ambercattle379
19 May 2018
School
University of Sydney
Department
Biological Sciences
Course
BIOL1003
Professor
Osu Lilje

For unlimited access to Class Notes, a Class+ subscription is required.

Define the relationship of genotype and phenotype
Genotype determines phenotype
-
e.g. Tt, TT, tt
○
Genotype - genetic makeup of an organism, specific allelic composition
-
Phenotype - set of detectable characteristics or traits of an organism, appearance
-
e.g. AA or Aa = normal pigmentation, aa = albinism phenotype
15a)
4:04 PM
15. Inheritance Page 1
Unlock document

This preview shows pages 1-3 of the document.
Unlock all 19 pages and 3 million more documents.

Already have an account? Log in
Define alleles, heterozygosity and homozygosity
Each chromosome contains genes
-
Each gene codes for characteristic, determines proteins in a cell
-
Genes - discrete hereditary factors that determine traits
-
Locus - position of gene on a chromosome
-
Allele
Different forms of a gene on homologous chromosomes
-
e.g. Aa, aa, AA
-
Dominant allele - produces normal enzymes, appears in phenotype
-
Recessive - produces abnormal enzyme, does not appear in phenotype
-
Heterozygous
2 alleles are slightly different for one trait
-
e.g. Aa
-
Homozygous
2 alleles are identical for one trait
-
e.g. aa
-
Genome
All genes in one homologous set of 23 chromosomes in one individual
-
15b)
11:07 PM
15. Inheritance Page 2
Unlock document

This preview shows pages 1-3 of the document.
Unlock all 19 pages and 3 million more documents.

Already have an account? Log in
Describe meiosis and the consequences of recombination
During meiosis, chromosomes distributed so each gamete receives only one chromosome
from each homologous pair
-
Recombination
Pair of sex chromosomes separates so each secondary oocyte receives one X chromosomes,
whereas each sperm cell receives either an X or Y chromosome
-
Genes on a given chromosome linked, so tend to be inherited as a set rather than as
individual genes because chromosomes segregate during meiosis
○
Homologous chromosomes may exchange genetic info by CROSSING OVER in prophase
○
Resulting zygote has 47 or 46 chromosomes instead of 46
▪
Segregation errors may occur, as homologous pair may not segregate e.g. one daughter
cell receives both chromosomes, while the other receives none
○
Random distribution of genes
-
15c)*
Monday, 5 June 2017
1:15 PM
15. Inheritance Page 3
Unlock document

This preview shows pages 1-3 of the document.
Unlock all 19 pages and 3 million more documents.

Already have an account? Log in

Document Summary

Genotype - genetic makeup of an organism, specific allelic composition e. g. tt, tt, tt. Phenotype - set of detectable characteristics or traits of an organism, appearance e. g. aa or aa = normal pigmentation, aa = albinism phenotype: inheritance page 1. Each gene codes for characteristic, determines proteins in a cell. Genes - discrete hereditary factors that determine traits. Locus - position of gene on a chromosome. All genes in one homologous set of 23 chromosomes in one individual: inheritance page 2. During meiosis, chromosomes distributed so each gamete receives only one chromosome from each homologous pair. Pair of sex chromosomes separates so each secondary oocyte receives one x chromosomes, whereas each sperm cell receives either an x or y chromosome. Genes on a given chromosome linked, so tend to be inherited as a set rather than as individual genes because chromosomes segregate during meiosis. Homologous chromosomes may exchange genetic info by crossing over in prophase.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

BIOL1003 Lecture Notes - Lecture 2: Anaerobic Respiration, Cellular Respiration, Ribosomal Rna
ambercattle379

Related Questions

Expression vectors inprokaryotes do not make functional eukaryotic gene products inbacteria very well because

Answer Not sure which one ?!

· the codon sequence for prokaryotes is differentthan the codon sequence in eukaryotes

· there are no disulfide bridges formed in proteinsnormally made in prokaryotes

· prokaryotic expression vectors cannot translateeukarytic sequences

· eukaryotic genes have introns, and prokaryotesdon't

· eukaryotic genes have exons and prokaryotesdon't


I create a knockout mouseusing the agouti/black fur system \. When I cross the agoutioffspring of the originally engineered mouse, I find a ratio of 2agouti mice to 1 black furred mouse. What is the bestexplanation?

Answer not sure which one?!

· The gene knocked out was recessive.

· The gene knocked out was dominant.

· The gene knocked out was a lethalgene.

· The knockput was integrated into a random spot,and did not knockput the original gene.

A restriction enzyme cuts DNAand leaves the following end

xxCTGCA
xxG

Which of the following could be the sequence of the correspondingend of the other end of the cut DNA?

Answer

· xxG
xxCTGCA

· xxC
xxGTGCA

· xxCCGAT
xxG

· xxGGCTA
xxC


An SNP always occurs dueto

Answer

· a one base-pair change.

· a three base-pair change.

· a single restriction site change.

· a single amino acid change.

· None of these.


I PCR out a mutated gene in apatient with Lisenbee chorea (the inability to dance in acoordinated fashion) and I compare it to another patient with thatsame disease phenotype. One subject had a mutation on chromosome 4,and the other subject couldn't rock it because of a mutation onchromosome 5. This is an exampe of

Answer

· pleitropy

· locus heterogeneity

· allelic heterogeneity

· dominant negative mutation

· penetrance

In his experiments, Mendelnoted that when two traits are involved in a genetic cross, theyare inherited independently of each other. Though Mendel didn'tknow about chromosomes, this still holds true (mostly)because

Answer

· genes on the same chromosome are often mixedthrough meiotic crossover during the formation ofgametes.

· variant alleles of the same gene do not interferewith the phenotypic expression of the gentoype in different geneticloci.

· most higher organisms are diploid, making only twoalleles available for every genetic locus, and there is a 50/50chance of geting either allele from a single parent.

· expression of phenotypes can vary from thegenotypes because of encironmantal influences.

· chromosomes often recombine

Anticipation is caused by amutation that increases in expressivity over subsequentgenerations.

Answer

· True

· False

Question 44

I have a genotype that shouldproduce a specific phenotype, but some of the individuals with thegenotype do not demonstrate any evidence of the phenotype. I wouldconsider this an example of

Answer

· allelic heterogeneity.

· locus heterogeneity.

· penetrance.

· expressitivity.

· sheer luck.

Question

Which of the following doesnot occur during the PCR reactions?

Answer

· Changes in the reaction temperature by athermocycler

· Denaturation at high temperatures

· Synthesis of oligonucleotide primers

· Extension of primers by DNA polymerase

· Annealing of oligonucleotide primers

Question

DNA markers, or variantnon-coding regions of DNA, are DNA polymorphisms that are usefulfor genetic mapping.

Answer

· True

· False

A mosaic is an organismwith

Answer

· multiple genotypes within one organism

· multiple alleles within one genotype

· more than one color of fur

· transgenes added to the zygote beforedevelopement

· a wt phenotype but a mutated genotype

Question

Genotype causesphenotype.

Answer

· No, gentoype just influences phenotype.

· Yes, genotype is the DNA sequence that createsphenotype.

Question

A true genetic chimera can becreated by

Answer

· mutating a gene early on in the development of anorganism resulting in different alleles being present in theadult

· multiple fertilized eggs or zygotes fusing to formone embryo

· adding a transgene to the genome of an organismduring fetal development only

· adding cells of a different species to an adultorganism

· adding a transgene to the genome of an animal atany stage in development

Question

The ABO blood group can bestbe explained by the concept of _______.

Answer

· dominant traits

· recessive traits

· allelic heterogeneity

· locus heterogeneity

· vampirism

If a loss of functionmutation creates a dominant phenotype, it may be becauseof

Answer

· haploinsufficiency

· penetrance

· expressivity

· allelic heterogeneity

· locus heterogeneity

Please select the best matchfor each.

Answer

· PCR: Technique used to generatecopies of a DNA fragment

· Southern blot : Screeningprocedure used to assay DNA fragments

· RFLPs : DNA fragments generatedby restriction enzymes that vary in length betweenindividuals

· Northern blot : Screeningprocedure used to assay mRNA fragments

· Electrophoresis Movement ofmacromolecules in an electrical field

Answer

A.

Screening procedure used toassay DNA fragments

B.

Movement ofmacromolecules in an electrical field

C.

Screening procedure used toassay mRNA fragments

D.

DNA fragments generated byrestriction enzymes that vary in length betweenindividuals

E.

Technique used to generatecopies of a DNA fragment

A gene mutates, and theprotein produced has a novel way of interacting with the cell, andcreates a new phenotype because of this new functionality. This iscalled

Answer

· pleitropy

· locus heterogeneity

· allelic heterogeneity

· dominant negative mutation

· gain of function dominant mutation

Question 62

Mutations in the somaticchromosomes may be inherited by the next generation.

Answer

· True

· False


A degenerate PCR primer withmany variant sequences must be used to make multiple copies of agene

Answer

· if only the protein sequence of the gene productis known to construct the primers

· if the DNA you are using is cDNA to constructthe primers

· if the DNA you are using is genomic DNA to becopied

· if the DNA you are trying to copy iscDNA

· if the vector is prokaryotic and the transformedcell is eukarytotic

Question

Please select the best matchfor each term.

Answer

· Restriction enzyme


· PCR C

· Plasmid

· YAC A

· Dideoxynucleotide

Answer

A.

Endonuclease isolated frombacteria

B.

Eukaryotic cloningvector

C.

Method for amplification ofDNA sequences

D.

Small circular fragment ofDNA

E.

Molecule used in DNAsequencing

Question

A couple goes on MauryPovitch, and the results are in: you are not the father. But noother man impregnated the female (granted, unlikely for a MauryPovitch guest, but work with me here) and he must be the father.DNA analysis claims otherwise, though the child definitely wasmom's (poor thing). What may be going on here?

Answer

· the child is parthenogenic because the motheractually impregnated herself like a shark, and the child's DNA isall mom's

· the child had a mutation that changed the genethat is used to trace paternity

· the child is a mosaic because he is actually a setof twins fused early during fetal development, and therefore camefrom 2 eggs and 2 sperm

· the dad may have germ-line mosaicism, meaning thatthe genotype of his sperm is different from his somaticgenotype

· mitochondrial DNA only comes from mom, so there isno way to tell whobthe baby's father is




Question 1: For each of the following terms, select the best definition:

gene, phenotype, chromosome, genotype, allele, genome

2.DNA that provides the instructions for synthesizing various types of small RNA molecules1.The complete set of genetic material in a cellan observable trait (or traits) of an organism

3.DNA that provides instructions for building a proteina variant of a gene

4.DNA of no known function

5.The genetic composition that underlies a trait (or traits)

6.A single molecule of DNA & its associated "helper" molecules

7.The portion of DNA that controls when and where proteins are synthesized

Question 2: Fill in the blank with these terms: dependent variable, independent variable, y-axis, x-axis

Understanding graphs is an important skill in science, since being able to visually depict data is often useful for identifying patterns, and can sometimes be the only tool for easily evaluating whether or not the data supports your hypothesis. When viewing a graph, the______ is plotted on the______, and the______we are measuring is plotted on the_____. For example, in one of the photosynthesis experiments last week, temperature was the______, while the size of the bubble at the end of the experiment was the_____

Question 3: For each of the following terms, select the best definition:

diploid cell, haploid cell, fertilization, zygote, gamete, somatic cell

1. The merging of two gametes

2. Any cell in an organism, except those used for reproduction

3. A cell with only one copy of each gene

4. The process of cell division

5. The cells used for sexual reproduction (generally eggs & sperm)

6. The initial cell of an organism

7. A cell that is capable of differentiating into multiple types of specialized cells

8. A cell with two copies of each gene

Question 1: For each of the following terms, select the best definition:

gene, phenotype, chromosome, genotype, allele, genome

2.DNA that provides the instructions for synthesizing various types of small RNA molecules1.The complete set of genetic material in a cellan observable trait (or traits) of an organism

3.DNA that provides instructions for building a proteina variant of a gene

4.DNA of no known function

5.The genetic composition that underlies a trait (or traits)

6.A single molecule of DNA & its associated "helper" molecules

7.The portion of DNA that controls when and where proteins are synthesized

Question 2: Fill in the blank with these terms: dependent variable, independent variable, y-axis, x-axis

Understanding graphs is an important skill in science, since being able to visually depict data is often useful for identifying patterns, and can sometimes be the only tool for easily evaluating whether or not the data supports your hypothesis. When viewing a graph, the______ is plotted on the______, and the______we are measuring is plotted on the_____. For example, in one of the photosynthesis experiments last week, temperature was the______, while the size of the bubble at the end of the experiment was the_____

Question 3: For each of the following terms, select the best definition:

diploid cell, haploid cell, fertilization, zygote, gamete, somatic cell

1. The merging of two gametes

2. Any cell in an organism, except those used for reproduction

3. A cell with only one copy of each gene

4. The process of cell division

5. The cells used for sexual reproduction (generally eggs & sperm)

6. The initial cell of an organism

7. A cell that is capable of differentiating into multiple types of specialized cells

8. A cell with two copies of each gene