BCMB2901 Lecture Notes - Lecture 5: G1 Phase, Dna Replication, S Phase

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Lecture 5 & 6: Eukaryotic DNA replication
Eukaryotic replication
Copying linear chromosomes
Genome much bigger than E. coli genome
Occurs in cell cycle
Cell cycle
Initiation of replication
DNA must be replicated only once in cell cycle
Make sure: enough of these origins are activated so each chromosome is completely copied
Ensure: replication is not initiated from an origin after it has been copied
Two phases of replication initiation
1. Replicator selection: origins selected
Not all origins are used in each round of replication
Replicator selection occurs during G1 phase
Formation of pre-replicative complexes (pre-RCs)
Pre-RCs sit at the origins until cells pass from G1 to S phase
2. Origin activation: replication machinery is recruited
Once S phase has begun, assembled pre-RCs are activated to initiate replication
Phosphorylation of pre-RCs promotes recruitment of DNA polymerases
G1 = gap
S = synthesis
G2 = gap
M = mitosis
G0 = state of withdrawal from cell
cycle (non-dividing cells)
Interphase = time between end of
one mitosis & start of the next (i.e.
G1, S, G2)
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