300833 Lecture Notes - Lecture 7: Macconkey Agar, Methylene Blue, Eosin Y

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14 Nov 2018
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Methylene blue agar and mannitol salt agar: macconkey agar: in macconkey agar , the selective components in it are bile salts and crystal violet , which inhibit the growth of gram-positive bacteria. These dyes also react with acidic products released by certain gram-negative bacteria when they use lactose or sucrose as carbon and energy sources. In this reaction colonies of gram-negative bacteria produce large amounts of acidic products that have a green metallic sheen: mannitol salt agar: in mannitol salt agar a concentration of 7. 5% Pathogenic staphylococci can be differentiated based on the release of acidic products when they use mannitol as a carbon and energy source. The acidic products cause a ph indicator in the medium to turn yellow. Counting chambers: counting chambers consist of specifically designed slides and coverslips, the space between the slide and coverslip creates a chamber of known depth.

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